Molecular Cloning of a cDNA Encoding a Cathepsin B Homologue from the Mulberry Longicorn Beetle, Apriona germari

  • Kim, Seong-Ryul (College of Natural Resources and Life Science, Dong-A University) ;
  • Yoon, Hyung-Joo (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology) ;
  • Park, Nam-Sook (College of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Sang-Mong (Department of Sericultural and Entomological Biology, Miryang National University) ;
  • Moon, Jae-Yu (College of Agriculture and Life Sciences, Seoul National University) ;
  • Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University) ;
  • Sohn, Hung-Dae (College of Natural Resources and Life Science, Dong-A University)
  • 발행 : 2002.03.01

초록

A cDNA encoding a putative member of cathepsin B of the thiol pretense superfamily was cloned from a cDNA library of the mulberry longicorn beetle, Apriona germari. Sequence analysis of the cDNA encoding the cathepsin B of A. germari (AgCatB) revealed that the 972 bp cDNA has an open reading frame of 324 amino acid residues. The deduced protein sequence of the AgCatB showed high homology with cathepsin B of the insects, Bombyx mori (47.3% amino acid identity), Helicoverpa armigera (46.6%) and Sarcophaga peregrina (45.6%), and the lowest homology with Aedes aegypti (33.2%). The AgCatB contains six disulfate bonds typical for cysteine pretenses. The three amino acid positions Cys-109, His-267, and Asn-287 which are conserved, active sites characteristic for cathepsin B, were also found. Phylogenetic analysis further confirmed that the AgCatB has a close relationship with that of B. mori, H. armigera and S. peregrina.

키워드

참고문헌

  1. Bechet, D. M., M. J. Ferrara, S. B. Mordier, M. P. Roux, C. D. Deval and A. Obled (1991) Expression of lysosomal cathepsin B during calf myoblast-myotube differentiation. Characterization of a cDNA encoding bovine cathepsin B. J. Biol. Chem. 266, 14104-14112
  2. Chan, S. J., B. San Segundo, M. B. McCormick and D. F. Steiner (1986) Nucleotide and predicted amino acid sequences of cloned human and mouse preprocathepsin BcDNAs. Proc. Natl. Acad. Sci. USA 83, 7721-7725 https://doi.org/10.1073/pnas.83.20.7721
  3. Cho, W. L., S. M. Tsao, A. R. Hays, R. Walter, J. S. Chen, E. S. Snigirevskaya and A. S. Raikhel (1999) Mosquito cathepsin B-like protease involved in embryonic degradation of vitellin is produced as a latent extraovahan precursor. J. Biol. Chem. 274,13311-13321 https://doi.org/10.1074/jbc.274.19.13311
  4. Crowson, R. A. (1981) The biology of the Coleoptera. Academic Press. London
  5. Daly, H. V., J. T. Doyen and A. H. Purcell III (1998) Introduction to insect biology and diversity. 2nd edition, Oxford University Press, London
  6. Guenette, R. S., M. Mooibroek, K. Wong, P. Wong and M. Tenniswood (1994) Cathepsin B, a cysteine protease implicated in metastatic progression, is also expressed during regression of the rat prostate and mammary glands. Eur. J. Biochem. 226, 311-321 https://doi.org/10.1111/j.1432-1033.1994.tb20055.x
  7. Kim, S. R., H. J. Yoon, N. S. Park, S. M. Lee, J. Y. Moon, B. R. Jin and H. D. Sohn (2001) Molecular cloning of a cDNA encoding a cathepsin D homologue from the mulberry longicorn beetle, Apriona german, Int. J. Indust. Entomol. 3,121-126
  8. Kwon, J. Y., F. Prat, C. Randall and C. R. Tyler (2001) Molecular characterization of putative yolk processing enzymes and their expression during oogenesis and embryogenesis in rainbow trout (Oncorhynchus mykiss). Biol. Reprod. 65, 1701-1709 https://doi.org/10.1095/biolreprod65.6.1701
  9. Kyte, J. and R. F. Doolittle (1982) A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157, 105-132 https://doi.org/10.1016/0022-2836(82)90515-0
  10. Merckelbach, A., S. Hasse, R. Dell, A. Eschlbeck and A. Ruppel (1994) cDNA sequences of Schistosoma japonicum coding for two cathepsin B-like proteins and Sj32. Trop. Med. Parasitol. 45, 193-198
  11. Musil, D., D. Zucic, D. Turk, R. A. Engh, I. Mayr, R. Huber, T. Popovic, V. Turk, T. Towatari, N. Katunuma and W. Bode (1991) The refined 2.15 A X-ray crystal structure of human liver cathepsin B: the structural basis for its specificity. EMBO J. 10, 2321-2330
  12. Swofford, D. L. (1990) PAUP: phylogenetic analysis using parsimony, ver. 3.0. Illinois Natural History Survey, Champaign (on disk)
  13. Takahashi, N., S. Kurata and S. Natori (1993) Molecular cloning of cDNA for the 29 kDa proteinase participating in decomposition of the larval fat body during metamorphosis of Sarcophaga peregrina (flesh fly). FEBS Lett. 334, 153-157 https://doi.org/10.1016/0014-5793(93)81702-2
  14. Xu, Y. and H. Kawasaki (2001) Isolation and expression of cathepsin B cDNA in hemocytes during metamorphosis of Bombyx mori. Comp. Biochem. Physiol. B. 130, 393-399 https://doi.org/10.1016/S1096-4959(01)00448-1
  15. Yoon, H. J., J. S. Bae, I. Kim, B. R. Jin, Y. I. Mah, J. Y. Moon and H. D; Sohn (2001) A phylogenetic study in some long horned beetle (Coleoptera: Cerambycidae) using mitochondrial COI gene and 16S rRNA sequences, Int. J. Indust. Entomol. 2, 37-53
  16. Yoon, H. J. and Y. I. Mah (1999) Life cycle of the mulberry longicorn beetle, Apriona germari Hope on an artificial diet. J. Asia-Pacific Entomol. 2, 169-173 https://doi.org/10.1016/S1226-8615(08)60046-1
  17. Yoon, H. J., Y. I. Mah, I. G. Park, S. B. Lee and S. Y. Yang (1997) The mode of hibernation of mulberry longicom beetle, Apriona germari Hope in Korea. J. Seric. Sci. Jpn. 66, 128-131
  18. Zhao, X. F., J. X. Wang and Y. C. Wang (1998) Purification and characterization of a cysteine proteinase from eggs of the cotton boll worm, Helicoverpa armigera. J. Insect Biochem. Mol. Biol. 28, 259-264 https://doi.org/10.1016/S0965-1748(98)00015-0