KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Expression of Plasma Membrane $H^ +-ATPase $ in the Roots of Plants Under Low Temperature

바이오센서에 의한 뿌리 원형질막에서의 $H^ +-ATPase $의 활성측정

  • 천병수 (연세대학교 의과대학) ;
  • 정갑채 (전남대학교 생물공학연구소) ;
  • 유종수 (한국해양대학교 해양과학기술연구소) ;
  • Published : 2002.02.01
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Abstract

The enzyme sensor for ATPase activity consisted of an immobilized membrane of two enzymes, purine nucloside phosphorylase (NP) and xanthine oxidase (XOD), and oxygen electrode. The $H^ +-ATPase $ rate of the plasma membranes increased by low root temperature. A cucumber and a pumpkin plasma membrane $H^ +-ATPase $$ activities measured by the proposed sensor system were in good agreement with the results obtained by a conventional UV spetrometer assay. One cycle of assay could be completed within 3 minutes.

본 연구에서는 특히 환경변화에 민감한 오이, 호박을 선택하여 저온 스트레스에 의한 기작 반응을 인지하는 이온 채널들의 식물에 가해지는 수송능력의 변화에 따른 능동 운송과 스트레스 감응 능력에 따른 생리학적 규명을 위해 시료 처리가 복잡하고 장시간 소요되는 종래법에서 탈피하고자 바이오센서에 의한 진속, 간단, 경제적 측정법을 도입하였다. 시료한 개를 측정하는데 걸리는 시간은 3분이 소요되며 센서법과 종래법과의 측정결과 사이에 좋은 상관치를 나타내었다.

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References

  1. Physiol. Plant v.94 NaCl-induced accumlation of tonoplast and plasma membrane $H^+$-ATPase message in tomato Binzel, M. L. https://doi.org/10.1111/j.1399-3054.1995.tb00990.x
  2. Plant Cell Physiol. v.36 Effect of root zone temperature on the mineral composition of xylem sap and plasma membrane $K^+$-$Mg^{2+}$-ATPase activity of grafted-cucumber and-figleaf gourd root systems Choi, K. J.;G. C. Chung;S. J. Ahn
  3. J. Biol. Chem. v.264 Structure of a plasma membrane $H^+$-ATPase gene from the plant. Arabidopsis thaliana Pardo, J. M.;R. Serrane
  4. Physiol. Plant v.103 The association of actin and tubulin with plasma membranes:Charecterization using inside-out vesicles formed by Brij 58 Sonesson, A.;S. Widell https://doi.org/10.1034/j.1399-3054.1998.1030308.x
  5. Physiol. Plant v.93 The induction of freezing tolerance in jack pine seeding:The role of root plasma membrane $H^+$-ATPase and redox activities Zhao, S.;S. T. Colombo.;E. Blumwald https://doi.org/10.1034/j.1399-3054.1995.930109.x
  6. Fisheries Science v.62 no.6 Development of sensor for ATPase activity Cheun, B. S.;H. D. Endo.;T. H. Hayashi.;E. Watanabe https://doi.org/10.2331/fishsci.62.950
  7. Korean J. Biotechnol. Bioeng. v.11 no.5 Determination of $K^+$, $CA^{2+}$ and $Mg^{2+}$ ATPase activities in Fish Muscle protein by ATPase Biosensor Cheun, B. S.;H. K. Kim.;E. H. Lee.;E. Watanabe
  8. Sci. Hortic. v.3 Crop production in recirculating nutrient solution Cooper, A. J. https://doi.org/10.1016/0304-4238(75)90008-4