생명과학회지 (Journal of Life Science)

  • 제12권2호
  • /
  • Pages.182-187
  • /
  • 2002
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

알킬화제인 MMS를 선처리한 NIH3T3 세포에서 소목 추출물 의한 세포고사의 촉진

Extracts of Caesalpina sappan L. Potentiate the Apoptosis of NIH3T3 Cells Exposed to Methymethane Sulfonate

  • 박종군 (원광대학교 자연대 생명과학부) ;
  • 황성진 (원광대학교 자연대 생명과학부) ;
  • 이정섭 (조선대학교 자연대 생물과학부) ;
  • 전병훈 (원광대학교 한의과대학) ;
  • 김원신 (원광대학교 자연대 생명과학부)
  • 발행 : 2002.04.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 알킬화제인 methylmethane sulfonate (MMS)를 선처리한 NIH3T3세포에서 소목추출물의 효과를 분석하였다. MTT 분석결과 MMS에 의해서 유도된 세포생존률이 소목추출물에 의해서 감소되었다. 세포형태분석, acridine orange 염색법, 그리고 DNA fragmentation 분석에서 MMS에 의해서 유도된 세포고사의 특징인 핵 응축 및 DNA laddering이 소목추출물에 의해서 증가됨이 관찰되었다. 이러한 결과들로 소목추출물은 NIB73T3 세포에서 MMS에 의해서 유도된 세포고사를 촉진시킴을 보여준다.

In this study we have investigated the effect of Caesalpina sappann L. extracts on the apoptosis in NIH3T3 cells exposed to methylmethan sulfonate (MMS), an alkylating agent. MTT assay study showed that Caesalpina sappan L. extracts potentiate the MMS-induced viability. Cell morphology studies, acridine orange (AO) staining, and DNA fragmentation analysis indicated that the postincubation of Caesalpina sappan L. extracts increase the nuclear condensation of MMS-induced apoptotosis. These results suggest that Caesalpina sampan L. extracts contain components potentiating MMS-induced apoptosis of NIH3T3 cells.

키워드

참고문헌

  1. Cancer Res v.48 Feasibility of drug screening with panels of human tumor cell lines using a microculture terazolium assay Alley;M. C.;M. L. Hursey;R. H. Shoemarker;J. G. Czerwinski;D. L. Fine;B. J. Abbott
  2. Planta Medica v.63 no.5 Brazillin modulates immine function mainly by augmenting T cell activity in halothane adminstered mice Choi;S. Y.;K. M. Yang;S. D. Jeon;L. Y. Kim;T. S. Chang;C. K. Moon https://doi.org/10.1055/s-2006-957722
  3. extract. Kor. J. Pharmacogn. v.30 no.1 In vitro studies on the anticancer effect and topoisomerase I inhibition activity of Caesalpinia sappan L. Jeon;W. K.;K. J. Park;S. Y. Kim;J. Y. Ma;H. J. Sung
  4. Method Enwymol v.194 Classical mutagenesis techinques Lawrence;C. W. https://doi.org/10.1016/0076-6879(91)94021-4
  5. J. Food Sci. and Technol. v.9 Antimicrobial activity of ethanol of Caesalpina sappan L. and Effect of the extract on the fermentation of Kimchi Lee;S. H.;W. J. Choi;O. K. Jo;S. J. Son.
  6. Kor. J. Food Sci. Tenhnol. v.28 no.1 Antioxidative activity of some solvent extract from Caesalpinia sappan L. Lim;D. K.;L. U. Choi;D. H. Shin
  7. Chung-Kuo Chung Hsi I Chieh Ho Tsa Chih v.12 no.10 Treatment of intestinal metaplasia and atypical thperplasia of gastric mucosa with xiao wei yan powder Liu;X. P.;W. Q. Han;D. R. Sun.
  8. Drug and Chemical Toxicology v.15 no.1 Brazilin protects cultured rat hepatocytes from BrCCI3-induced toxicity Moon;C. K.;K. S. Park;S. G. Kim;H. S. Won;J. H. Chung https://doi.org/10.3109/01480549209035174
  9. Chem. Pharm. Bull v.35 no.9 Homoisoflavoids and related compound. Ⅳ. Absolute comfigurations of homoisoflavonoids from Caesalpinia sappan L. Namikosi;M.;T. Saitoh
  10. Planta Medica v.65 no.5 Anticomplementary activity of comstituents from the heartwood of Caesalpinia sappan Oh;S. R.;D. S. Kim;I. S. Lee;K. Y. Jung;J. J. Lee;H. K. Lee https://doi.org/10.1055/s-2006-957481
  11. Kor. J. Pharmacogn v.28 no.4 Cytotoxic effectof Korean traditional prescription on the human gastric cancer cell lines Park;K. J.;E. H. Kim;Y. A. Eun;B. J. Kang;H. J. Sung
  12. J. Bacterial v.171 Generation of an endogenous DNA-methylating agent by nitrosation in Escherichia coli Taverna;P.;B. Sedgwichk
  13. Life Sci. v.67 Vasorelaxing effectsof Caesalpinia sappan involvement of endogenous nitric oxide Xie;Y. W.;D. S. Ming;H. X. Xr;H. Dong;P. P. But https://doi.org/10.1016/S0024-3205(00)00772-4
  14. 중약대사전 김창민;신민교;이경순;안덕규
  15. 중약대사전 신문농출판공가
  16. 한약자원식물학 장상문;최정;김종원;박병윤;박선동
  17. 본초학 전국한의과대학 본초학교수

피인용 문헌

  1. Identification of catalytic acidic residues of levan fructotransferase from Microbacterium sp. AL-210 vol.17, pp.1, 2007, https://doi.org/10.5352/JLS.2007.17.1.006
  2. Effect of fisetin on UVB-induced apoptosis and DNA single strand breaks in NIH3T3 cells vol.17, pp.1, 2007, https://doi.org/10.5352/JLS.2007.17.1.064