Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Protective Effect of Jaboyangyeong-hwan Water Extracts on CCl4-Induced Liver Damage

자보양영환의 물추출물이 사염화탄소로 유발된 간 손상에 미치는 영향

  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 이형철 (원광대학교 한의과대학 병리학교실) ;
  • 황상구 (원광대학교 한의과대학 병리학교실) ;
  • 남은영 (원광대학교 한의과대학 병리학교실) ;
  • 김대근 (원광대학교 한의과대학 병리학교실) ;
  • 박정원 (원광대학교 한의과대학 병리학교실) ;
  • 이영찬 (원광대학교 한의과대학 병리학교실) ;
  • 박승택 (의과대학 해부학교)
  • Published : 2002.04.01
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Abstract

Jaboyangyeong-hwan (IAE) has been known as a traditional medicine for the treatment of debility, fatigue, and liver diseases. The hepatoprotective effect of the water extract of Jaboyangyeong-hwan was investigated against carbon tetrachloride ($CCl_4$)-induced hepatic damage. A single intraperitoneal injection of $CCl_4$produced liver damage in rats as manifested by the significant rise of aspartate aminotransferase (AST, alanine aminotransferase (ALT), and alkaline phosphatase (ALP) in serum as compared to those of untreated normal group. Pretreatments of rats with the JAE extract (300, 600, and 1200 mg/kg for 7 days) were significantly reduced AST, ALT, and ALP levels compared with $CCl_4$-treated control group. Treatment of rats with $CCl_4$led to significantly increase in lipid peroxidation and significantly decrease in cytochrome P450 and P450 reductase. The oral administration of the JAE extract significantly inhibited the accumulation of microsomal thiobarbituric acid reactive substance (TBARS) and increased the cytochrome P450 and P450 reductase activity. All these biochemical alterations resulting from $CCl_4$administration were inhibited by the pretreatment with JAE extract. These results suggest that JAE water extract can be useful as a hepatoprotective agent.

자보양영환은 자보폐간의 효능이 있어 간허증상의 치료제로 사용되었던 전통약물로 잘 알려져 있다. 따라서 본 연구에서는 사염화탄소로 간 손상을 유도한 흰쥐에서 자보양영환의 물추출물이 간 보호효과가 있는지 알아보았다. 사염화탄소의 1회 복강투여는 혈청 aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP)의 유의적인 증가가 관찰되어 사염화탄소에 의한 간 손상이 유발되었음을 알 수 있었다. 자보양영환 물 추출물(300, 600, and 1200 mg/kg, 7 days)을 전 처리한 횐쥐에서는 사염화탄소 단독 투여군인 대조군에 비해 AST, ALT, ALP의 유의한 감소양상이 관찰되었다. 또한 사염화탄소의 투여는 microsome의 지질과산화가 유도되었으며, 이물질대사효소(cytochrome P450 및 P450 reductase)의 유의한 감소가 확인되었다. 자보양영환 물추출물의 경구투여는 간의 지질과산화 지표인 microsome의 TBARS 생성을 억제하였으며 cytochrome P450 및 P450 reductase 활성도가 대조군에 비해 유의한 증가되어 정상수준으로 회복되었다. 자보양영환 추출물의 투여는 사염화탄소의 투여에 따른 생화학적 지표들의 변화를 억제하는데, 이러한 실험결과에서 자보양영환의 물추출물은 사염화탄소로 유도한 간 독성에 대하여 해독작용 및 간 보호효과가 있는 것으로 판단된다.

Keywords

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