KSBB Journal

  • 제17권2호
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  • Pages.182-188
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  • 2002
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  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

실관 생물반응기대 고정화된 Enterococcus faecalis RKY1에 의한 숙신산 생산특성

  • 위영중 (전남대학교 화학공학부 생물산업기술연구소) ;
  • 윤종선 (전남대학교 화학공학부 생물산업기술연구소) ;
  • 류화원 (전남대학교 화학공학부 생물산업기술연구소)
  • 발행 : 2002.04.01
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초록

푸마르산으로부터 숙신산 생물전환을 위하여 E. faecalis RKY1을 HFBR에 친정화하여 연속생산 공정에 대한 가능성을 모색하였다. E. faecalis RKY1은 실관의 spongy 부분에 효과적으로 고정화되었으며, transverse mode로 HFBR 조업시 실관가닥이 50개 및 200개일 경우 거의 비슷한 경향을 나타냈다. 또한 배지의 공급속도를 0.25, 0.5, 1.0 mL/min로 증가시키면서 조업한 결과, 정상상태에 도달하는 시간이 각각 24, 12, 9시간으로서 유속이 증가할수록 정상상태에 도달하는 시간은 단축되었지만, 반응기 내의 기질 및 생성물 분포는 그 변화가 심하였다. 실관 생물반응기를 이용하여 숙신산 생물 전환시 배지의 공금 유속이 증가할수록 숙신산 생산성은 증가하였지만 전환수율이 감소하여 미반응 푸마르산은 증가하였다. 최대 숙신산 생산성 및 이 때의 수율은 푸마르산염 농도 80 g/L 및 배지 공금 유속 2.0 mL/min일 때 17.1 g/L ·hr 및 0.54 g/g이었으며, 최적 숙신산 생산성 및 이 때의 수율은 푸마르산염 농도 50 g/L 및 배지 공급 유속 1.0 mL/min일때 9.0 g/L · 및 0.90 g/g으로서 회분식 생물전환의 경우보다 더 우수하였다.

Enterococcus faecalis RKY1 cells were immobilized in an asymmetric hollow fiber bioreactor for application to the continuous production of succinic acid. The media was fed into shell-side of the module using a peristaltic pump, and the products were collected through lumen-side outlet. The number of hollow fibers within the module did not affect the bioreactor efficiency in the transverse operated hollow fiber bioreactor. The steady state at the outlet of hollow fiber bioreactor was reached after 24 hr cultivation at flow rate of 0.25 mL/mim, 12 hr at 0.5 L/min, and 9 hr at 1.0 mL/mm, respectively. The succinate and fumarate concentrations within the hollow fiber bioreactor, however, were as changeful as increasing the flow rate. During continuous operation with the flow rates between 0.5 and 2.0 mL, the productivity of succinate was 8.0-10.9 g/L $.$ hr at 30 g/L fumarate, 4.9-14.9 g/L hr at 50 g/L fumarate, and 7.2-17.1 g/L hr at 80 galL fumarate, respectively.

키워드

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