Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Specific bovine antibody response against a new recombinant Cryptosporidium parvum antigen containing 4 zinc-finger motifs

  • De Graaf, Dirk-C. (Veterinary and Agrochemical Research Centre) ;
  • Coninck, Hans-De (Veterinary and Agrochemical Research Centre) ;
  • Petry, Franz (Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University Mainz) ;
  • Eeckhout, Ilka-B. (Veterinary and Agrochemical Research Centre) ;
  • Peeters, Johan-E. (Veterinary and Agrochemical Research Centre)
  • Published : 2002.03.01
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Abstract

A Cryptosporidium parvum sporozoite and oocyst λgt11 cDNA library was screened with a hyperimmune rabbit serum that was developed against insoluble fragments of ultrasonicated oocysts. A clone named Cp22.4.1 encoding a protein of 231 amino acids with 4 zinc-finger domains characterized by a Cys-X2-Cys-X4-His-X4-Cys motif was isolated and characterized. There was a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD-TOPO-TA expression vector permitted to evaluate the antigenicty of the recombinant His-tagged antigen. This antigen was recognized by 2 out of 5 sera from Cruptosporidium immune calves and not by sera from parasite naive animals.

Keywords

References

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