Identification of newly isolated Babesia parasites from cattle in Korea by using the Bo-RBC-SCID mice

  • Cho, Shin-Hyeong (Department of Medical Zoology, National Institute of Health) ;
  • Kim, Tong-Soo (Department of Medical Zoology, National Institute of Health) ;
  • Lee, Hyeong-Woo (Department of Medical Zoology, National Institute of Health) ;
  • Tsuji, Masayoshi (School of Veterinary Medicine, Rakuno Gakuen University) ;
  • Ishihara, Chiaki (School of Veterinary Medicine, Rakuno Gakuen University) ;
  • Kim, Jong-Taek (Animal Teaching Hospital, Kangwon National University) ;
  • Wee, Sung-Hwan (National Veterinary Research and Quarantine Service) ;
  • Lee, Chung-Gil (College of Veterinary Medicine, Chonnam National University)
  • Published : 2002.03.01

Abstract

Attempts were made to isolate and identify Korean bovine Babesia parasite. Blood samples were collected from Holstein cows in Korea. and Babesia parasites were propagated in SCID mice with circulating bovine red 1)food cells for isolation. The isolate was then antigenically and genotypically compared with several Japanese isolates. The Korean parasite was found to be nearly identical to the Oshima strain isolated from Japanese cattle, which was recently designated as Babesia ovata oshimensis n. var. Haemaphusalis longicornis was the most probable tick species that transmitted the parasite .

Keywords

References

  1. Arai S, Tsuji M, Kim SJ, Nakada T, Kanno Y, Ishihara C (1998) Babesia canis infection in canine-red blood cell-substituted SCID mice. Int J Parasitol 28: 1429-1435
  2. Bosma GC, Custer RP, Bosma MJ (1983) A severe combined immunodeficiency mutation in the mouse. Nature 301: 527-530
  3. Han TW, Kim SK, Jeon Y (1966) The classification and distribution of Korean cattle tick. Rural Res Report, RDA 9: 91-103. (in Korean)
  4. Higgins DG, Bleasby AJ, Fuchs R (1992) Clustal V: improved software for multiple sequence alignment. Comput Appl Biosci 8: 189-191
  5. Jeon Y (1978) Identification of a strain of Babesia isolated from Korean cattle. Korean J Vet Res 18: 27-31. (in Korean)
  6. Laemmli, DK (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227: 680-685
  7. Lee HB, Choi WP (1976) Field case of babesiasis in Korean native cattle. Korean J Vet Res 16: 173-177. (in Korean)
  8. McCune JM, Namikawa R, Kaneshima H, Schultz LD, Lieberman M, Weissman IL (1988) The SCID-hu mouse: murine model for the analysis of human hematolymphoid differentiation and function. Science 241: 1632-1639
  9. Medlin L, Elwood HJ, Stickel S, Sogin ML (1988) The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71: 491-499
  10. Minami T, Ishihara T (1980) Babesia ovata sp. n. isolated from cattle in Japan. Natl Inst Anim Health Q 20: 101-113.(in Japanese)
  11. Ohta M, Tsuji M, Tsuji N, Fujisaki K (1995) Morphological, serological and antigenic characteristics, and protein profile of newly isolated Japanese bovine Babesia parasite with particular reference to those of B. ovata. J Vet Med Sci 57: 671-675
  12. Ohta M, Kawazu S, Terada Y, Kamio T, Tsuji M, Fujisaki K (1996) Experimental transmission of Babesia ovata oshimensis n. var. of cattle in Japan by Haemaphysalis longicornis. J Vet Med Sci 58: 1153-1155
  13. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Evol 4: 406-425
  14. Sanger F, Nicklen S, Cloulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci 74: 5463-5467
  15. Soulsby EJL (1982) Helminths, arthropods and protozoa of domesticated animals. 7th ed. pp 456-475 & 706-728, Bailliere Tindall, London
  16. Suh MD (1987) Pure isolation and identification of Babesia ovata by morphological characteristics and complement fixation test in Korea. Korean J Vet Res 27: 307-316. (in Korean)
  17. Terada Y, Tsuji M, Hagiwara K, Takahashi K, Ishihara C (1995) Clearance of Theileria sergenti-infected bovine red blood cells in severe combined immunodeficency mice. Vet Parasitol 60: 221-228
  18. Tsuji M, Hagiwara K, Takahashi K, Ishihara C, Azuma I, Siddiqui WA (1992) Theileria sergenti proliferates in SCID mice with bovine erythrocyte transfusion. J Parasitol 78: 750-752.
  19. Tsuji M, Terada Y, Arai S, Okada H, Ishihara C (1995) Use of the Bo-RBC-SCID mouse model for isolation of a Babesia parasite from grazing calves in Japan. Exp Parasitol 81: 512-518