Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antimicrobial Characteristics of Yellow-Pigment Produced by Monascus anka Y7

Monascus anka Y7이 생성하는 황색소의 항균 특성

  • 이호재 (동의공업대학 식품생명과학과) ;
  • 박미연 (부경대학교 수산식품연구소)
  • Published : 2002.04.01
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Abstract

Antimicrobial activity of yellow pigment produced by Monascus anka Y7 (Y7) was studied. The crude yellow pigment of Y7 showed antimicrobial activity against some bacteria and yeasts. The diameter of inhibition zone against gram-positive bacteria was a little smaller t]fan that of gram-negative bacteria to the crude yellow pigment. Especially, E2 fraction obtained from the crude yellow pigment by TLC method showed high anti-microbial activity against E. coli.. The fraction had bright yellow pigment, showing fluorescent light and having the maximum absorption at 373 nm. Citrinin, a mycotoxin which had been characterized as an antimicrobial substance from a Monascus strain, was not detected in the E2 fraction and in the crude yellow pigment by the results of TLC and HPLC. This indicates that the antimicrobial activity of Y7 pigments did not any relationship with citrinin. Yellow degree (b/a of Hunters color value) of Y7 pigment was much higher than that of other natural colorants such as annatto, gardenia yellow and carthamus yellow. But the colors of all of the yellow pigments were similar by panels. Thus, the yellow pigment of Y7 could be used as a useful alternative colorant for food industry, having the advantage of antimicrobial activity.

Monascus anka Y7이 생성하는 황색소의 항균활성 특성을 규명하였다. Y7의 조황색소는 그람 양성균보다 그람 음성균에 대한 항균 활성이 더 크게 나타났으며 Candida 등과 같은 효모에 대해서도 항균활성을 나타냈다. 특히 황색소의 항균성분 중 TLC로 분리한 E2 분획은 E. coli에 대한 항균활성이 크며 373 nm에서 주된 흡광 특성을 보이고 있고 흡수 파장 범위가 장파장 부위로 넓어져 있어 외관상 진한 밝은 황색을 나타내는 형광색소이다. Y7의 조색소 및 각 TLC 분획의 HPLC분석 결과 citrinin이 검출되지 않았다. 이는 Y7의 항균 특성이 최근 Monascus 색소에서 안전성의 논란이 되고 있는 citrinin과는 무관한 것을 의미한다. 한편 E2 분획을 주로하는 조황색소는 식품에 일반적으로 사용되고 있는 천연황색소인 치자황색소, 아나토색소, 홍화색소보다 황색도(b/a)가 매우 높고 이들 색소와 유사한 색조를 나타내므로 항균성을 보유한 대체 황색소로서의 가치가 높을 것으로 사료된다.

Keywords

References

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