Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Protective Immune Reponses Induced by Non-infectious L-particles of Equine Herpesvirus Type-1: Implication of Cellular Immunity

  • Published : 2002.03.01
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Abstract

Mice immunized with equine herpesvirus type-1(EHV-1) L-particles skewed a significant increase (p<7.75) in serum antibody titers. Upon a booster dose four weeks lateral antibody titers increased significantly. Interestingly, immunization via intravenous or intramuscular route induced significantly higher (p<0.75) antibody titers. However, mice iummunized with UV-treated L-particles, visions or immunization via intranasal route induced lower antibody titers. Upon challenge inoculation with wildtype EHV-1, our data showed there was a poor correlation between antibody titers and protection against virus replication. Therefore, the role of cell-mediated immunity Inwards protection was investigated. As predicted, the strongest cell-mediated immunity, as measured by delayed-hypersensitivity test, was detected in mice immunized with live virus particles. The magnitude of cell-mediated immune response correlated with the efficacy of L-particles as immunizing agent. The highest efficacy, as indicated in mice immunized via intranasal routed was highly correlated with cell-mediated immunity. A similar phenomenon was also demonstrated in mice immunized intranasally with UV-treated L-particles. However, the degree of protection was reduced when mice immunized intravenously or intramuscularly with UV-treated L-particles. In conclusion, protection conferred in these animals was highly implicated by immune cells and the least by antibodies. The route of immunization and the nature of the antigen also contributed to the efficacy of L-particles as immunizing agent. In contrast to that of herpes simplex virus type 1, our data showed EHV-1 non-infectious L-particles are highly suitable for immunization of the host against EHV-1 disease.

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References

  1. Res. Vet. Sci. v.59 Role of T cells, virus neutralizing antibodies and complement-mediated antibody lysis in the murine response against equine herpesvirus type-1 (EHV-1) infection of C3H (H-2k) and BALB/c-(H-2d) mice Alber,D.G;J.Greensil;R.A.Killington;A.Stokes https://doi.org/10.1016/0034-5288(95)90003-9
  2. Prog Vet. Microb. Immun v.2 Molecular epizootiology, pathogenesis and prophylaxis of equine herpesvirus-1 infection Allen,G.P;J.T.Bryans
  3. J. Virol. v.69 Major histocompatibility complex class 1-restricted cytotoxic T-lymphocyte responses in horses infected with equine herpesvirus 1 Allen,G.;M. Yeargan;L.R.Costa;R.Cross
  4. J. Vet. Malaysia v.7 Class and subclass of antibody responses to equine herpesvirus type-1 (EHV-1) in the serum and respiratory secretions of infected-mice Azmi,M
  5. Journal of General Virology v.74 Cell-mediated antiviral response to EHV-1 in a murine model by means of adoptive transfer of immune spleen cells Azmi,M;H.J.Field https://doi.org/10.1099/0022-1317-74-2-275
  6. Bull. WHO v.54 The enzyme linked immunosorbent assay (ELISA) Bidwell D.E;A.A.Buck;H.J.Deisfeld;B.Enders;J.Haworth;G.Hudldt;N.H.Kent;C.Kirsten;P.Mattern;E.J.Ruitenberg;A.Voller
  7. Vet. Quart v.12 Viraemia and abortions are not prevented by 2 commercial equine herpesvirus-1 vaccines after experimental challenge of horses Burke,F.W;Rossmanith;N.Nowotny;C.Pallan;K.Mostl.H.Lussy https://doi.org/10.1080/01652176.1990.9694249
  8. Veterinary Record v.114 Trials of an inactivated equid herpesvirus-1 vaccine: challenge with subtype-2 virus Burrows,R;D.Goodridge;M.S.Denyer https://doi.org/10.1136/vr.114.15.369
  9. J. Virol. Meth v.15 A method for the quantitative analysis of ELISA Data Clark,M.F;D.J.Barbara https://doi.org/10.1016/0166-0934(87)90099-1
  10. Vaccine v.8 Protection against lethal equine herpesvirus type-1 (subtype-1) infection in hamsters by immune stimulating complexes (ISCOMS) containing the major viral glycoproteins Cook,R.F;T.Oneill;E.Strachan;B.Sundquist;J.A.Mumford https://doi.org/10.1016/0264-410X(90)90252-H
  11. Archives of Virology v.123 Pathogenesis of equine herpesvirus-1 in specific pathogen-free foals:primary and secondary infections and reactivation Gibson,J.S;J.D.Slater;A.R.Awan;H.J.Field https://doi.org/10.1007/BF01317269
  12. Gene v.87 Characterization of the gene and an antigenic determinant of equine herpesvirus type-1 glycoprotein-14 with homology to gB-equivalent glycoproteins of other herpesviruses Guo,P https://doi.org/10.1016/0378-1119(90)90309-F
  13. Virology v.130 Isolation and characterization of a non-infectious virion-like particle released from cells infected with human strains of cytomegalovirus Irmiere,A;W.Gibson https://doi.org/10.1016/0042-6822(83)90122-8
  14. J. Gen. Virol. v.72 Characterizaion of envelopend tegument structures(L-particles) produced by alphaherpesviruses:integrity of the tegument does not depend on the presene of capsid or envelope Mclauchlan,J;F.J.Rixon
  15. Virology v.190 Non-infectious L-paticles supply functions which can facilitate infection by HSV-1 Mclauchlan,J;C.Addison;M.C.Craigie;F.J.Rixon https://doi.org/10.1016/0042-6822(92)90906-6
  16. Avi. Path v.11 Enzyme-linked immunosorbent assay(ELISA) for detecting infectious laryngotracheitis viral antibodies in chicken serum Meulemans,G;P.Halen
  17. Virology v.208 Protection against EHV-1 challenge infection in the murine model after vaccination with various formulations of recombinant glycoprotein gp 14 (gB) Osterrieder,N;R.Wagner;C.Brandmuller;P.Schmidt;H.Wolf;O.R.Kaaden https://doi.org/10.1006/viro.1995.1181
  18. Can. J. Comp. Med. v.42 The protective antigen of equine herpesvirus type-1 Paap-Vid,G;J.B.Derbyshire
  19. Canadian Journal of Comparative Med v.43 The virus neutralizing activity of antibodies specific to the envelope and nucleoscapsid of equine herpesvirus type-1 Paap-Vid,G;J.B.Derbyshire
  20. J. Gen. Virol v.73 Assembly of enveloped tegument structures (L-particles) can occur independently of virion maturation in herpes-simplex virus type 1-infected cells Rixon,F.J;C.Addison;J.Mclauchlan https://doi.org/10.1099/0022-1317-73-2-277
  21. Arch. Virol. v.123 The family Herpesviridae: an update Roizman,B;R.C.Desrosiers;B.Fleckenstein;C.Lopez;A.C.Minson;M.J.Studdert https://doi.org/10.1007/BF01317276
  22. Vet. Immun. and Immunopath v.67 Equine herpesvirus type 1 infects dendritic cells in vitro: stimulation of T lymphocytes proliferation and cytotoxicity by infected dendritic cells Siedek, E.M;M.Whelan, N. Edington;A. Hamblin https://doi.org/10.1016/S0165-2427(98)00203-7
  23. Arch. Virol. v.104 Monoclonal antibodies with neutralizing activity to equine herpesvirus 1 Shimizu,M;K.Satou;N.Nishioka https://doi.org/10.1007/BF01313819
  24. J. Gen. Virol v.70 A Hamster model of equine herpesvirus type-1(EHV-1) infection-passive protection by monoclonal-antibodies to EHV-1 glycoprotein-13, glycoprotein-14 and Glycoprotein-17/18 Stokes,A;P.K.Murray;G.P.Allen;L.A.Pullen https://doi.org/10.1099/0022-1317-70-5-1173
  25. Virus Research v.44 The production of a truncated form of baculovirus expressed EHV-1 glycoprotein C and its role in protection of C3H (H-2K K)mice against virus challenge Stokes,A;D.G.Alber;R.C.Cameron;R.N.Marshal;G.P.Allen;R.A.Killington https://doi.org/10.1016/0168-1702(96)01339-1
  26. J. Gen. Virol. v.72 Identification and characterization of a novel non-infectious herpes-simplex virus-released particle Szilagyi,J.F;C.Cunningham https://doi.org/10.1099/0022-1317-72-3-661
  27. Virology v.189 The DNA sequence of equine herpesvirus-1 Telford,E.A.R;M.S.Warson;K.McBride;A.J.Davison https://doi.org/10.1016/0042-6822(92)90706-U
  28. J. Gen. Virol v.75 Characterization of immune responses to baculovirus expressed equine herpesvirus 1 glycoprotein D and H in a murine model Tewari,D;J.M.Whalley;D.N.Love;H.J.Field https://doi.org/10.1099/0022-1317-75-7-1735
  29. Arch. Virol. v.140 Immunization with glycoprotein C of equine herpesvirus-1 is associated with accelerated virus clearance in a murine model Tewari,D;S.V.Nair;M.De Ungria;G.L.Lawrence;M.Hayden;D.N.Love;H.J.Field;J.M.Whalley https://doi.org/10.1007/BF01309967
  30. Manual of clinical immunology Enzyme linked immunosorbent assay, in research Voller,A;D.Bidwell;A.Barlett;H.Friedman(ed)