Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Seasonal Variation of Eubacterial Community Structure and Their Structure Affecting Environmental Parameters in Reservoir

남매지에서 Eubacteria 군집구조의 계절적 변화와 그에 영향을 미치는 환경요인

  • 이희순 (대구대학교 생명과학부) ;
  • 박정원 (영남대학교 해양과학연구소) ;
  • 김미경 (영남대학교 해양과학연구소) ;
  • 이영옥 (대구대학교 생명과학부)
  • Published : 2002.03.01
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Abstract

For elucidating the correlation between the eubacterial community structure and environmental parameters in Nammae Reservoir located in Kyungsan, Kyungbuk, the bacterial community structure and their structure affecting environmental parameters were analyzed using Fluorescent In Situ Hybridization (FISH) monthly over year. $\alpha$ . $\beta$ . $\gamma$-subclasses of Proteobacteria and Cytophaga-Flavobacterium (CF) group known as dominant bacterial group in freshwater were detected in 3 stations over year. The ratio of each subclass to total bacteria was determined; $\alpha$.$\beta$ . $\gamma$-subclasses and CF group varied in the range of 4.0~29.2%, 1.7~25.8%, 1.8~12.8%, 4.9~36.3%, respectively and there was no substantial differences between stations. In terms of the correlation between each group specific bacteria and environmental parameters such as temperature, SS, pH, DOC, NH$_4$-N, NO$_3$-N, PO$_4$-P, standing crops of algae, the results were as follows: 1) total bacterial numbers correlated positively with temperature, SS and DOC, 2) Eubacteria positively with DOC and Chl-$\alpha$, 3)${\gamma}$-subclass positively with DOC, and 4) CF group positively with standing crops of chlorophyceae, 5) whereas $\beta$-subclass bacteria correlated negatively with standing crop of cyanobacteria and that of total algae.

경북 경산시 에 위치한 남매지 에서 세균군집 의 구조를 fluorescent in situ hybridization(FISH) 방법을 이용하여 분석하였고 아울러 세균군집크기에 영향을 주는 환경요인을 측정하여 그 상관성을 파악하고자 하였다. 담수계에 우점하는 것으로 알려진 proteobacteria($\alpha$.$\beta$.$\gamma$-subclasses)와 글Cytophaga-Flavobacterium (CF) group에 속하는 진정세균군의 연중변화를 정점별로 측정하였다. 총세균수에 대한 각 세균군집 비율은 정점간의 큰 차이 없이 $\alpha$-subclass가 4.0~29.2%,$\beta$-subclass가 1.7~25.8%, $\gamma$-subclass가 1.8~12.8%를, 그리고 CF group은 4.9~36.3%의 군집 비율을 나타냈다. 온도,SS, pH, DOC,질산염, 조류 현존량 등 환경요인과 세균군집 간의 상관관계를 분석한 결과, 총세균수는 수온, DOC,55, eubacteria는 DOC, 녹조류 현존량, $\gamma$-subclass 세균군은 DOC, CF group세 균은 녹조류 현존량과 양의 상관성을 나타낸 반면 $\beta$-subclass는 남세균 . 총 조류 현존량과 음의 상관성을 나타냈다.

Keywords

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