Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Korean Wheat on LDL Oxidation and Atherosclerosis in Cholesterol-Fed Rabbits

고콜레스테롤혈증 유발 가토를 이용한 우리밀의 LDL산화 및 동맥경화 예방 효과

  • Choe, Myeon (Dept. of Animal Products Science, Kangwon National University) ;
  • Kim, Hyun-Sook (Institute of Korea Nutritional Science, Kangwon National University)
  • 최면 (강원대학교 축산가공학과) ;
  • 김현숙 (한국영양과학연구소)
  • Published : 2002.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

Numerous studies have suggested the involvement of oxidative processes in the pathogenesis of atherosclerosis and especially of oxidized low density lipoproteins (LDL). We studied the effect of Korean wheat on the oxidizability of LDL and the development of experimental atherosclerosis in rabbits on cholesterol diet. For the approach of the aim, antioxidative activity of wheat extracts against oxidation of LDL was investigated. The antiatherogenic effect of wheat was studied against Newzealand whithe (NZW) rabbits fed containing the wheat powder for 12 weeks. PBS extracts of Korean and imported wheats decreased LDL oxidation induced by copper ion by 62.3%, 52.6% respectively in comparison with control. Liver thiobarbituric acid reactive substance (TBARS ) levels of rabbits were significantly lowered in Korean wheat fed group (0.397$\pm$ 0.029 nmol MDA/mg protein, p<0.05) compared to control (0.496$\pm$0.021 nmol MDA/mg protein) and imported wheat group (0.478$\pm$0.019 nmol MDA/mg protein). TBARS levels of kidney showed no significant difference among treatment groups. The aorta of cholesterol-fed rabbits showed typical atherosclerotic lesions when compared with the controls, but the microscopic differences between groups was not clear. The present study suggests that Korean wheat may have higher antioxidative capacity than imported wheat and have more beneficial effects for the treatment of disease by inhibiting the oxidation of LDL.

우리밀과 수입밀의 PBS 추출물의 LDL 산화 억제 효과를 비교하였고, 고콜레스테롤혈증 유발 가토의 조직중 지질과 산화 및 대동맥의 조직 변화를 측정함으로써 우리밀이 동맥경화의 진행 양상 변화에 어떠한 효과가 있는지 검토하였다. 밀 추출물의 LDL 산화 억제 효과는 우리밀과 수입밀이 각각 62.5%, 52.8%로 나타나 우리밀 추출물의 LDL 산화 억제 효과가 수입밀에 비해 약간 높았다. 고콜레스테롤혈증 유발 가토의 간장중 TBARS 함량은 수입밀보다 우리밀 섭취시 낮게 나타나 우리밀이 지질 과산화를 억제하는데 효과가 있는 것으로 나타났지만, 신장에서의 억제 효능은 비슷한 것으로 나타났다. 대동맥의 석회 침착 발생율은 대조군에 비해 낮았지만 우리밀과 수입밀의 섭취로 인한 뚜렷한 차이는 입증되지 않았다. 이상의 결과로 추출물의 LDL 산화 억제 효과가 입증되었으며, 우리밀 섭취가 콜레스테롤 섭취로 인한 간장중 지질 과산화적 손상을 완화시키는데 기여함을 알 수 있었지만 우리밀과 수입밀 섭취로 인한 동맥경화 예방효과에 대한 뚜렷한 차이는 나타나지 않았다.

Keywords

References

  1. Lee, Y.G. : Cardiovascular disease, truely is that increased?J. Korean Med. Association, 35, 734-737 (1992)
  2. Cho, S.H. : Dietary lipid and atherosclerosis. J. Korean Soc. Food Sci. Nutr., 23, 170-179 (1994)
  3. Esterbauer, H., Gebicki, J., Pohl, H. and Jurgens, G. : The role of lipid peroxidation and antioxidants in oxidative modification of LDL. Free Radic. Biol. Med., 13, 341-390 (1992) https://doi.org/10.1016/0891-5849(92)90181-F
  4. Ishikawa, Y., Inadera, H., Shirai, K., Hashimoto, H., Fukamachi, I., Saito, Y. and Yoshida, S. : Moderate oxidation of hypertriglyceridemicLDL causes apolipoprotein-B epitope change and enhances its uptake by macrophages. Biochim. Biophys. Acta, 1129, 60-64 (1992)
  5. Ross, R. : The pathogenesis of atherosclerosis, perspective for the 1990's. Nature, 29, 801-809 (1993)
  6. Fogelman, A.M., Shechter, I., Sieger, J., Hook, M., Child, J.S. and Edward, P.A. : Malondialdehyde alteration of low density lipoprotein leads to cholesteryl ester accumulation in human monocyte-macrophages. Proc. Natl. Acad. Sci. USA, 77, 2214-2218 (1980) https://doi.org/10.1073/pnas.77.4.2214
  7. Morel, D.W., Hessler, J.R. and Chisolm, G.M. : Low density lipoprotein cytotoxicity induced by free radical peroxidation of lipids. J. Lipid Res., 24, 1070-1076 (1983)
  8. Fukudome, S., Jinsma, Y., Matsukawa, T., Sasaki, R. and Yoshikawa, M. : Release of opioid peptids, gluten exorphins by the action of pancreatic elastase. FEBS Lett., 412, 475-479 (1997) https://doi.org/10.1016/S0014-5793(97)00829-6
  9. Stein, G.M., Schaller, G., Pfuller, U., Wagner, M., Wagner, B., Schietzel, M., Bussing, A. and Krebsforschung, H. : Characterization of granulocyte stimulation by thionins from European mistletoe and from wheat. Biochim. Biophys. Acta., 1426, 80-90 (1999) https://doi.org/10.1016/S0304-4165(98)00139-1
  10. Vaaler, S., Hanssen, K.F., Dahl-Jorgensen, K., Frolich, W., Aaseth, J., Odegaard, B. and Aagenaes, O. : Diabetic control is improved by guar gum and wheat bran supplementation. Diabet. Med., 3, 230-233 (1986) https://doi.org/10.1111/j.1464-5491.1986.tb00750.x
  11. Lankisch, M., Layer, P., Rizza, R.A. and DiMagno, E.P. : Acute postprandial gastrointestinal and metabolic effects of wheat amylase inhibitor in normal, obese, and diabetic humans.Pancreas, 17, 176-181 (1998) https://doi.org/10.1097/00006676-199808000-00011
  12. Choe, M., Park, J.B. and Kim, H.S. : Screening of immuneenhancing substances from Korean wheats. J. Korean Soc. Food Sci. Nutr., 29, 307-311 (2000)
  13. Havel, R.J., Eder, H.A. and Bragdon, J.H. : The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J. Clin. Invest., 34, 1341-1353 (1955)
  14. Lowry, O.H., Rosebrough, N.J., Fan, A.L. and Randall, R.J. : Protein measurement with Folin phenol reagent. J. Biol. Chem., 193, 265-271 (1951)
  15. Buege, J.A. and Aust, S.D. : Microsomal lipid peroxidation. Methods Enzymol., 52, 302-310 (1978) https://doi.org/10.1016/S0076-6879(78)52032-6
  16. 허명회 : SAS 범주형 데이터 분석. 자유아카데미, 서울 (1989)
  17. Park, J.O., Kim, K.S., Ji, Y.A. and Ryu, B.H. : Antioxidant activity of daidzin and puerarin toward oxidation of human low density lipoprotein. J. Korean Soc. Food Sci. Nutr., 26, 25-31 (1997)
  18. Munday, J.S., James, K.A., Fray, L.M., Kirkwood, S.W. and Thompson, K.G. : Daily supplementation with aged garlic extract, but not raw garlic, protects low density lipoprotein against in vitro oxidation. Atherosclerosis, 143, 399-404 (1999) https://doi.org/10.1016/S0021-9150(98)00329-3
  19. Rosenblat, M., Belinky, P., Vaya, J., Levy, R., Hayek, T., Coleman, R., Merchav, S. and Aviram, M. : Macrophage enrichment with the isoflavan glabridin inhibits NADPH oxidase induced cell-mediated oxidation of low density lipoprotein. A possible role for protein kinase C. J. Biol. Chem., 14, 13790-13799 (1999)
  20. Sparrow, C.P. and Olszewski, J. : Cellular oxidative modification of low density lipoprotein dose not require lipoxygenase. Proc. Natl. Acad. Sci. USA, 89, 128 (1992)111111 https://doi.org/10.1073/pnas.89.1.128
  21. Rankin, S.M., Parthasarathy, S. and Steinberg, D. : Evidence for a dominant role of lipoxygenase(s) in the oxidation of LDL by mouse peritoneal macrophage. J. Lipid Res., 32, 449 (1991)
  22. Kwon, M.J., Song, Y.S. and Song, Y.O. : Antioxidative effect of kimchi ingredients on rabbits fed cholesterol diet. J. Korean Soc. Food Sci. Nutr., 27, 1189-1196 (1998)

Cited by

  1. Simultaneous Analysis of Bangpungtongseong-san and Its Antioxidant Effect vol.21, pp.2, 2013, https://doi.org/10.14374/HFS.2013.21.2.133
  2. Effects of grape pomace on the antioxidant defense system in diet-induced hypercholesterolemic rabbits vol.4, pp.2, 2010, https://doi.org/10.4162/nrp.2010.4.2.114
  3. 기능성 소재를 첨가한 우리밀 제빵 제품의 항산화 활성과 단백질 소화도 vol.24, pp.6, 2002, https://doi.org/10.17495/easdl.2014.12.24.6.853
  4. Comparison on the Quality Characteristics of Korean and Imported Wheat Including Chinese Wheat vol.28, pp.4, 2018, https://doi.org/10.17495/easdl.2018.8.28.4.317