한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제31권1호
  • /
  • Pages.92-97
  • /
  • 2002
  • /
  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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칡 열수추출물이 흰쥐의 알콜올 대사효소계에 미치는 영향

Effects of Pueraria thunbergiana Bentham Water Extracts on Hepatic Alcohol Metabolic Enzyme System In Rats

  • 김명주 (대구산업정보대학 식품영양과) ;
  • 이정수 (대구산업정보대학 식품영양과) ;
  • 하오명 (본초제약) ;
  • 장주연 (대구산업정보대학 식품영양과) ;
  • 조수열 (대구산업정보대학 식품영양과)
  • 발행 : 2002.02.01
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초록

갈화와 갈근이 알코올성 간손상 흰쥐의 알코올 대사효소계에 미치는 영항을 구명하기 위하여 알코올을 투여한 흰쥐에게 갈화와 갈근 열수추출물을 2수준으로 나누어 5주간 급여한 후 그 효과를 관찰하였다. 체중증가량, 식이섭취량과 식이효율은 알코올 투여시 감소하였으며 갈화와 갈근추출물 급여로 회복되는 경향을 보였다. 장기 무게에 미치는 칡추출물의 영향은 알코올 투여로 증가된 간, 뇌와 심장 무게를 감소시켰는데 갈화가 갈근에 비하여 뇌와 심장 무게의 감소효과가 큰 것으로 나타났다. 알코올 대사효소 활성 변화에서 알코올 투여시 유의적으로 증가된 ADH와 MEOS 활성은 칡추출물 급여시 감소된 반면 AlDH 활성은 대조군에서 유의적으로 감소되었는데 칡추출물 급여시 그 활성이 증가되었다. MAO 활성과 과산화수소 함량도 알코올 투여시 증가되었으며 갈화와 갈근 열수추출물 급여시 유의적으로 감소되었다. 이상의 결과에서 갈화와 갈근 열수추출물 급여는 알코올 중독된 흰쥐의 알코올 대사계 효소 활성 변화에 영향을 미쳐 알코올성 간손상 완화에 효과적일 것으로 사료된다.

The effects of Pueraria flos (PF) and Pueraria radix (PR) water extract on the hepatic alcohol metabolic enzyme activities were examined in rats that were orally administered ethanol (25% v/v, 5g/kg body weight/day) for 5 weeks. The PF and PR water extract were supplemented in a diet, based on 1.2 g or 2.4 g of raw PF or PR/kg body weight/body. Alcohol administration without the PF or PR supplementation significantly decreased net weight gain, feed intake and feed efficiency ratio. However. both dose of the PF of PR supplementation resulted in significant enhancement of growth and suppression of increased relative weight of liver, brain and heart by alcohol administration. Activities of hepatic alcohol dehydrogenase and microsomal ethanol oxidizing system were higher in the alcohol treated group than in the normal group, while aldehyde dehydrogenase activity was significantly lowered in the alcohol treated group. The hepatic metabolic enzyme activities altered by alcohol administration were normalized by both doses of PF or PR supplement. Hepatic monoamine oxidase activity and hydrogen peroxide, which were significantly higher in the alcohol treated group than in the normal group, were also decreased by the supplementation with either PF or PR. These results indicate that low-or high-supplementation of either water extract PF or PR may alleviate ethanol-induced hepatotoxicity by altering alcohol metabolic enzyme activities.

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참고문헌

  1. Keung, W.M., Lazo, O., Kunze, L. and Vallee, B.L. : Potentiation of bioavailability of daidzin by an extract of radix Puerariae. Proc. Natl. Acad. Sci. USA, 93, 4284-4288 (1996) https://doi.org/10.1073/pnas.93.9.4284
  2. Niho, Y., Yamazaki, T., Nakajima, Y., Itoh, H., Takeshita, T., Kinjo, J. and Nohara, T. : Pharmacological studies on Puerariae-flos. 1. The effects of Puerariae - flos on alcoholic metabolism and spontaneous movement in mice. Yakugaku Zasshi, 109, 424-431 (1989) https://doi.org/10.1248/yakushi1947.109.6_424
  3. Yamazaki, T., Nakajima, Y., Niho, Y., Hosono, T., Kurashige, T., Kinjo, J. and Nohara, T. : Pharmacological studies on Puerariae-flos. 3 Protective effects of Kakkalide on ethanolinduced lethality and acute hepatic-injury in mice. J. Pharmacy and Pharmacology, 49, 831-833 (1997) https://doi.org/10.1111/j.2042-7158.1997.tb06122.x
  4. Fujiwara, N., Suwa, Y., Yoshizumi, H., Yamatodani, A. and Wada, H. : Preventive effects of L-alanine and L-glutamic acid on acute toxicity of acetaldehyde in mice. Jpn. J. Alcohol Drug Dep., 23, 58-69 (1988)
  5. Keung, W.M. and Vallee, B.L. : Daidzin and daidzein suppress free-choice ethanol intake by syrian golden hamsters. Proc. Natl. Acad. Sci. USA, 90, 10008-100012 (1993) https://doi.org/10.1073/pnas.90.21.10008
  6. Lee, C.H. : Biological effects of Korean Puerariae radix catechins on liver functon in rats administered with ethanol. J. Food Sci. Nutr., 2, 138-143 (1997)
  7. Peters, T.J. : Ethanol metabolism. British Medical Bulletin, 38, 17-20 (1982) https://doi.org/10.1093/oxfordjournals.bmb.a071726
  8. Pikkarainen, P.H., Salaspuro, M.P. and Lieber, C.S. : A method for the determination of free acetaldehyde in plasma. Alcoholism, 3, 259-261 (1979) https://doi.org/10.1111/j.1530-0277.1979.tb05311.x
  9. Reeves, P.G., Nielse, R.H. and Fahey, G.C. : AIN-93 purified diets for laboratory rodents; final report of the American Institute of nutrition Ad Hoc Writing Committ on the reformation of the AIN-93 rodent diet. J. Nutr., 123, 1939-1951 (1993) https://doi.org/10.1093/jn/123.11.1939
  10. Fujii, M., Ohmachi, T., Sagami, I. and Watanabe, M. : Liver microsomal drug metabolism in ethanol - treated hamsters. Biochem. Pharmacol., 34, 3881-3884 (1985) https://doi.org/10.1016/0006-2952(85)90438-1
  11. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. : Protein measurement with folin phenol reagent. J. Biol. Chem., 193, 265-275 (1951)
  12. Bergmeyer, H.U. : Methods of enzymatic analysis. Academic Press, N.Y., p.28 (1974)
  13. Koivula, T. and Koivusalo, M. : Different form of rat liver aldehyde dehydrogenase and their subcellular distribution. Biochem. Biophys. Acta., 397, 9-23 (1975)
  14. Lieber, C.S. and DeCarli, L.M. : Ethanol oxidation by hepatic microsomes-adaptive increase after ethanol feeding. Science, 162, 917-918 (1968) https://doi.org/10.1126/science.162.3856.917
  15. Choi, J.W. : Studies on the effect of ginseng saponin on the pharmacological action and hepatic alcohol-metabolizing enzyme activity in alcohol-treated mice. Ph.D. Thesis, Yeungnam University (1983)
  16. Kalaria, T., Mitchell, M.J. and Harik, S.I. : Correlation of 1-methyl-4-phenyl-1,2,3,6,-tetrahyfropyrene neurotoxcity with blood-brain barrier monoamine oxidase activity. Proc. Natl.Acta. Sci., 84, 3521-3525 (1987) https://doi.org/10.1073/pnas.84.10.3521
  17. Hildebrant, A.G. and Roots, I. : Reduced nicotinamide adenine dinucleotide phosphate (NADPH)-dependent formation and breakdown of hydrogen peroxide during mixed function oxidation reactions in liver microsomes. Arch. Biochem. Biophys., 171, 385-397 (1975) https://doi.org/10.1016/0003-9861(75)90047-8
  18. Snedecor, G.W. and Cochrane, W.G. : Statistical methods. 6th., Iowa State University Press, Iowa, p.1 (1967)
  19. Oh, S.I., Kim, C., Chun, H.J. and Park, S.C. : Chronic ethanol consumption affects glutathione in rat liver. J. Nutr., 128, 758-763 (1998) https://doi.org/10.1093/jn/128.4.758
  20. Lieber, C.S. : Alcohol and the liver; metabolism of ethanol, metabolism effects and pathogenesis of injury. Acta. Med. Scand. Suppl., 703, 11-55 (1985)
  21. Lee, M.K. : Effect of dietary protein and fiber levels on brain ethanol metabolism and antioxidant system in ethanol-treated rats. Ph.D. Thesis, Yeungnam University (1997)
  22. Cho, S.S. : Effect of Galwhahaejungtang on the ethanol metabolism in mouse. M.S. Thesis, Wonkwang University (1989)
  23. Hawkins, R.D., Kalant, H. and Khanna, J.M. : Effects of chronic intake of ethanol metabolism. Can. J. Physiol. Pharma., 44, 241-257 (1966) https://doi.org/10.1139/y66-029
  24. Moon, J.O. and Yang, J.H. : Alteration of the aldehyde dehydrogenase activity by the chronic ethanol administration. Yakhak Hoeji, 40, 563-573 (1996)
  25. Takase, S., Takada, A., Yasuhara, M. and Tsutsumi, M. : Hepatic aldehyde dehydrogenase activity in liver disease with particular emphasis on alcoholic liver disease. Hepatology, 9, 704-709 (1989) https://doi.org/10.1002/hep.1840090508
  26. Vidal, F., Toda, R., Gutierrez, C., Montserrat, B., Fernandez-Muixi, F., Lorenzo, A. and Richart, C. : Influence of chronic alcohol abuse and liver disease on hepatic aldehyde dehydrogenase activity. Alcohol., 15, 3-8 (1998) https://doi.org/10.1016/S0741-8329(97)00073-6
  27. Teschke, R., Moreno, F. and Petrides, A.S. : Hepatic microsomal ethanol oxidizing system (MEOS) : Respective roles of ethanol and carbohydrats for the enhanced activity after chronic alcohol consumption. Biochem. Pharmacol., 30, 1745-1751 (1981) https://doi.org/10.1016/0006-2952(81)90004-6
  28. Lieber, C.S. : The influence of alcohol on nutritional status. Nutr. Res., 46, 241-254 (1988)
  29. Kishimoto, R., Fujiwara, I., Kitayama, S., Goda, K. and Nakata, Y. : Changes in hepatic enzymes activities related to ethanol metabolism in mice following chronic ethanol administration. J. Nutr. Sci. Vitaminol., 41, 527-543 (1995) https://doi.org/10.3177/jnsv.41.527
  30. Koivula, T. and Lindros, K.O. : Effect of long-term ethanol treatment on aldehyde and alcohol dehydrogenase activities in rat liver. Biochem. Pharmacol., 24, 1937-1942 (1975) 111 https://doi.org/10.1016/0006-2952(75)90378-0
  31. Ohnishi, K. and Lieber, C.S. : Reconstitution of the microsomal ethanol-oxidizing system. Qualitative and quantitative changes of cytochrome P-450 after chronic ethanol consumption. J. Biol. Chem., 252, 7124-7131 (1977)
  32. Cho, S.Y., Kim, M.J., Park, E.M., Park, J.Y. and Jang, J.Y. : Effect of Puerari Flos and Radix water extracts on free radical generating system in ethanol-treated rats. Kor. J. Gerontol., 11, 8-13 (2001)
  33. Sandri, G., Panfili, E. and Ernster, L. : Hydrogen peroxide production by monoamine oxidase in isolated rat brain mitochondria; It's effect on glutathione levels and $Ca^{2+}$ efflux. Biochem. Biophys. Acta., 1035, 300-305 (1990) https://doi.org/10.1016/0304-4165(90)90092-B
  34. Tipton, K.F. : The prosthetic groups of pig brain mitochondria monoamine oxidase. Biochem. Biophy. Acta., 159, 451-459 (1969)
  35. 정성광, 곽춘식 : Ethanol 중독 흰쥐에서 총담관결찰이 간의 monoamine oxidase 활성에 미치는 영향. 한국생화학회지, 25, 210-218 (1992)
  36. Mevedev, A.E., Kirkel, A.Z., Kamyshanskaya, N.S., Gorkin, V.Z. and Anokhina, I.P. : Influence of ethanol administration on the activity and compartmentaion of rat liver monoamine oxidases. Alcohol and Alcoholism, 30, 729-735 (1995)
  37. Keung, W.M. and Vallee, B.L. : Daidzin and its anti dipsotropic analogs inhibit serotonin and dopamine metabolism in isolated mitochondria. Proc. Natl. Acad. Sci., 95, 2198-2203 (1998) https://doi.org/10.1073/pnas.95.5.2198
  38. Antonenkov, V.D. and Panchenko, I.F. : Effect of chronic ethanol treatment under partial catalase inhibition on the activity of enzymes related to peroxide metabolism in rat liver and heart. Int. J. Biochem., 20, 823-828 (1988) https://doi.org/10.1016/0020-711X(88)90071-7
  39. Handler, J.A. and Thurman, R.G. : Catalase-dependent ethanol oxidation in perfused rat liver. Requirement for fatty acidstimulated H2O2 production by peroxisomes. Eur. J. Biochem., 176, 477-484 (1988) https://doi.org/10.1111/j.1432-1033.1988.tb14305.x
  40. Lelbach, W.K. : Liver damage in chronic alcoholism. Results of a clinical, clinical-chemical and biooptic-histological study in 526 alcoholic patients during a low calorie diet in an open. Acta. Hepatogastroenterol., 13, 321-349 (1966)
  41. Krikun, G. and Cederbaum, A.I. : Effect of chronic ethanol consumption on microsomal lipid peroxide. Role of iron and comparison between control. FEBS Lett., 208, 292-296 (1986) https://doi.org/10.1016/0014-5793(86)81035-3
  42. Thurman, R.G. : Induction of hepatic microsomal reduced nicotinamide, adenine dinucleotide, phosphate-dependent production of hydrogen peroxide by chronic prior treatment with ethanol. Mol. Pharmacol., 9, 670-675 (1973)
  43. Ekstrom, G. and Ingelman-Sunderberg, M. : Rat liver microsomal NADPH-supported oxidase activity and lipid peroxidation dependent on ethanol-inducible cytochrome P-450 (P450ⅡE1). Biochem. Pharmacol., 38, 1313-1319 (1989) https://doi.org/10.1016/0006-2952(89)90338-9
  44. Lieber, C.S. and DeCarli, L.M. : Ethanol oxidation by hepatic microsomes -adaptive increase after feeding. Science, 162, 917-918 (1968) https://doi.org/10.1126/science.162.3856.917
  45. Lieber, C.S. : Alcohol and the liver 1994 update. Gastroentero., 106, 1085-1104 (1994) https://doi.org/10.1016/0016-5085(94)90772-2

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