한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제31권1호
  • /
  • Pages.21-25
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  • 2002
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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당-단백질 가열반응 시에 생성되는 향기성분에 미치는 지질의 영향

Effect of Lipid Mediated Glucose-Protein Reaction on Thermal Flayer Generation

  • 주광지 (계명대학교 식품영양학과)
  • 발행 : 2002.02.01
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초록

당-단백질의 가열반응 시에 생성되는 향기성분의 형성에 지질의 산화생성물이 어떠한 영향을 미치는가를 알아보고자 casein과 glucose(CG) 그리고 지질을 첨가한 사료(CGL)를 6$0^{\circ}C$에서 각각 2, 4주씩 저장한 후에 pressure reactor에 넣어 16$0^{\circ}C$에서 60분 가열 반응시킨 시료에서 향기성분을 추출하였다. Casein, glucose, 지질(CGL) 시료에서 단백질과 환원당의 반응물이 지질의 산화생성물과 반응하여 생성된 함질소 헤테로 고리화합물인 pyrazine, methylpyrazine과 2,5-dimethylpyrazine, 2-ethyl-5-methylpyrazine과 2-acetylpyrrole이 확인되었다. 또한 지질의 가열산화 분해성분인 3-methyll-butanol, heptanal, nonanal, 2-heptanone과 짧은 사슬의 지방산인 heptanoic acid, octanoic 가 검출되었다. 또 다른 화합물로 2-acetylfuran이 검출되었으며 glucose의 분해산물인 5-hdroxymethyl-2-furfural은 aems 시료에서 가장 큰 peak로 그 함량이 현저하게 많았다. 이상의 결과로 보아 시료에 첨가된 지질은 당-단백질 가열반응 시 생성되는 향기성분의 수와 그 함량을 증대시키는 작용을 하였다고 할 수 있다

The contribution of lipid to thermal flayer generation from glucose-protein reaction was accomplished by isolating flavor compounds from casein-glucose (CG)and casein-glucose-coin oil (CGL) which were stored for 2 and 4 weeks at 6$0^{\circ}C$ and then reacted at 16$0^{\circ}C$ for 1hr. The volatiles from the reactant mixtures were isolated by a solvent extraction method with methylene chloride and analyzed by gas chromatography and gas chromatography-mass spectrometry. Pyrazine, methylpyrazine, 2,5-dimethylpyrazine, 2-dimethylpyrazine ,2-ethy-5- methyIpyrazine and 2-acetylpyrrole originated from interaction of thermal degradation of casein and lipid oxidation were identified in the CGL samples. It was also found that 3-methyl-1-butanol, 2-cyclopene-1,4-diode, heptanal, nonanal, and 2-heptanone were derived from lipid source. Two additional fatty acids, heptanoic acid and octanoic acid were also identified in the CGL samples. 5-Hydroxymethyl-2-furfural, the most abundant volatile, was responsible for the formation of sugar degradation product. The results suggested that the presence of lipid in the samples had more effect on the contribution of volatile formation of glucose-protein thermal reaction than the absence of lipid in the samples.

키워드

참고문헌

  1. Pocorny, J. : Browning from lipid-protein interaction. Proc.Food Nutr. Sci., 5, 421-428 (1981)
  2. Ho, C-T. and Carlin, J.T. : Formation and aroma characteristics of heterocyclic compounds in foods. In Flavor chemistry: Trend and development, Teranish, R., Buttery, R.G. and Shahidi, F. (eds.), ACS Symposium Series 388, Amer. Chem. Soc., Washington DC., p.92-104 (1989)
  3. Hwang, S-S., Carlin, J.T., Bao, Y., Hartman, T.G. and Ho, C-T. : Characterization of volatile compounds generated from the reactions of aldehydes with ammonium sulfide. J. Agric. Food Chem., 34, 538-542 (1986) https://doi.org/10.1021/jf00069a042
  4. Rizzi, G.P., Steimle, A.R. and Patton, D.R. : Formation of dialkylthiophens in Maillard reactions involving cysteine. In Food Science and Human Nutrition, Charalambous, G. (ed.), Elsevier Science Publishers, Amsterdam, p.731-741 (1992)
  5. Zhang, Y. and Ho, C-T. : Volatile compounds formed from thermal interaction of 2,4-decadienal with cysteine and glutathione. J. Agric, Food Chem., 37, 1016-1020 (1989) https://doi.org/10.1021/jf00088a044
  6. AOCS : AOCS Official Method Cd 8-53. In Official Methods and Recommended Practices of the American Oil Chemists' Society, 4th ed., AOCS, Champaign (1990)
  7. Majlat, P., Erdos, Z. and Takacs, J. : Calculation and application of retention indices in programmed temperature gas chromatography. J. Chromatogr., 91, 89-103 (1974) https://doi.org/10.1016/S0021-9673(01)97888-4
  8. Huang, T-C., Bruechert, L.J. and Ho, C-T. : Kinetic of pyrazine formation in amino acid-glucose systems. J. Food Sci., 54, 1611-1614 (1990) https://doi.org/10.1111/j.1365-2621.1989.tb05172.x
  9. Hwang, H-I., Hartman, T.G., Rosen, R.T. and Ho, C-T. : Formation of pyrazines from the Maillard reaction of glucose and glutamine-amide-$^{15}N$. J. Agric. Food Chem., 41, 2112-2115 (1993) https://doi.org/10.1021/jf00035a054
  10. Lee, S-H. and Joo, K-J. : Analysis of volatile flavor compounds in sesame oil extracted by purge-and-trap method. Korean J. Food Sci. Technol., 30, 260-265 (1998)
  11. Yaylayan, V.A. and Keyhani, A. : Carbohydrate and amino acid degradation pathways in L-Methionine/D-[$^{13}$-C] Glucose model system. J. Agric. Food Chem., 49, 800-803 (2001) https://doi.org/10.1021/jf000986w
  12. Chiu, E.-M., Kuo, M.-C., Bruechert, L.J. and Ho, C-T. : Substitution of pyrazines by aldehydes in model system. J. Agric. Food Chem., 38, 58-61 (1990) https://doi.org/10.1021/jf00091a012
  13. Ho, C-T., Lee, M-H. and Chang, S.S. : Isolation and identification of volatile compounds from roasted peanuts. J. Food Sci., 47, 127-133 (1981)
  14. Izzo, H. and Ho, C-T. : Effect of residual amide content on aroma generation and browning in heated gluten-glucose model system. J. Agric. Food Chem., 41, 2364-3267 (1993) https://doi.org/10.1021/jf00036a028
  15. Shibamoto, T. : Volatile flavor chemicals formed by the Maillard reaction. In Thermal Generation of Aromas, Parliment, T.H., McGorrin, R.J. and Ho, C-T. (eds.), ACS Symposium Series 409, Amer. Chem. Soc., Washington DC., p.135-141 (1989)
  16. Zhang, Y., Dorjpalam, B. and Ho, C-T. : Contribution of peptides to volatile formation in the Maillrad reaction of casein hydrolysate with glucose. J. Agric. Food Chem., 40, 2467-2471 (1992) https://doi.org/10.1021/jf00024a026
  17. Yaylayan, V.A. and Keyhani, A. : Origin of 2,3-pentanedione and 2,3-butanedione in D-Glucdse/L-Alanine Maillard model system. J. Agric. Food Chem., 47 3280-3284 (1999) https://doi.org/10.1021/jf9902292
  18. Huang, T-C., Bruechert, L.J., Hartman, T.G., Rosen, R.T. and Ho, C-T. : Effect of lipids and carbohyderates on thermal generation of volatiles from commercial zein. J. Agric. Food Chem., 35, 985-990 (1987) https://doi.org/10.1021/jf00078a030
  19. Selke, E., Rohwedder, W.K. and Dutton, H.J. : Volatile components from tristearin heated in air. J. Amer. Oil Chem. Soc., 52, 232-235 (1975) https://doi.org/10.1007/BF02639148