Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Rapid Sex Identification of Chicken by Fluorescence In Situ Hybridization Using a W Chromosome-specific DNA Probe

  • Sohn, S.H. (Department of Animal Science and Technology, Graduate School, RAIRC, Jinju National University) ;
  • Lee, C.Y. (Department of Animal Science and Technology, Graduate School, RAIRC, Jinju National University) ;
  • Ryu, E.K. (Department of Animal Science and Technology, Seoul National University) ;
  • Han, J.Y. (Department of Animal Science and Technology, Seoul National University) ;
  • Multani, A.S. (Department of Cancer Biology, The University of Texas M. D. Anderson Cancer Center) ;
  • Pathak, S. (Department of Cancer Biology, The University of Texas M. D. Anderson Cancer Center)
  • Received : 2002.05.14
  • Accepted : 2002.07.05
  • Published : 2002.11.01
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Abstract

It has been known that the sex of chicken cells can be most accurately identified by fluorescence in situ hybridization (FISH). However, the presently available FISH has not been widely used for sex identification, because the procedures for cell preparation and FISH itself are complicated and time-consuming. The present study was undertaken to test a rapid FISH procedure for sexing chicken. A FISH probe was simultaneously synthesized and labeled with digoxigenin by polymerase chain reaction (PCR) targeting a 416 bp segment of the 717 bp XhoI family fragment which is repeated over 10 thousand times exclusively in the W chromosome. Sexing by FISH was performed on cytological preparations of early embryos, adult lymphocytes and feather pulps of newly hatched chicks. The DNA probe hybridized to all types of uncultured interphase as well as metaphase female but not male cells that had been examined. Moreover, consistent with the known site of the XhoI family, the hybridization signal was localized to the pericentromeric region of the W chromosome. We, therefore, conclude that the present PCR-based FISH can be used as a rapid and reliable sex identification procedure for chicken.

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References

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