Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Isolation of Candidate Nek2-Interating Protein Genes(NIPs)

  • Published : 2002.06.01
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Abstract

Nek2 is a mammalian protein kinase that is structurally homologous to NIMA, a mitotic regulator in Aspergillus nidulans. We recently observed that the Nek2 protein was localized in multiple sites within a cell in a cell cycle state-specific manner. This suggests that Ndk2 is involved in diverse cellular functions during the cell cycle progression. To have a better understanding on cellular functions in which Nek2 participates, we carried out yeast two-hybrid screening and isolated six candidate clones whose products interact with Nek2. Most of Nek2-interacting proteins (NIPs) appear cytoplasmic, suggesting that Nek2 is involved in cellular functions in cytoplasm. Further experiments are under progress to confirm their interactions with Nek2 and to understand their biological significance.

Keywords

References

  1. Nucleic Acids Res v.29 Leucine zipper motif of chicken histone acetyltrans-ferase-1 is essential for in vivo and in vitro interactions with thw p48 subunit of chicken chromatin assembly fator-1. Ahmad A;Nagamatsu N;Kouriki H;Takami Y;Nakayama T https://doi.org/10.1093/nar/29.3.629
  2. Trends Cell Biol v.9 The Aurora/lpl1p kinase family:regulators of chromosome segregation and cytokinesis. Bischoff JR;Plowman GD https://doi.org/10.1016/S0962-8924(99)01658-X
  3. Trends Cell Biol v.11 Coiled coils:a highly versatile protein foding mitif. Burkhard P;Stetefeld J;Strelkov SV https://doi.org/10.1016/S0962-8924(00)01898-5
  4. Cell v.102 Mitotic histone H3 phosphorylation by the NIMA kinase in Aspergillus nidulans. De Souza CP;Osmani AH;Wu LP;Spotts JL;Osmani SA https://doi.org/10.1016/S0092-8674(00)00035-0
  5. Development v.129 The MAPK pathway triggers actvation of Nek2 during chromosome condensation in mouse spermatocytes. Di Agostino S;Rossi P;Geremia R;Sette C
  6. EMBO J v.17 A centrosomal function for the human Nek2 protein hinase, a member of the NIMA famaily of cell cycle regulators. Fry AM;Meraldi P;Nigg EA https://doi.org/10.1093/emboj/17.2.470
  7. J Cell Biol v.141 C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2. Fry AM;Mayor T;Meraldi P;Stierhof YD;Tanaka K;Nigg EA https://doi.org/10.1083/jcb.141.7.1563
  8. J Cell Sci v.113 The NIMA-related kinase X-Nek2B is required for efficient assembly of the zygotic centrosome in Xenopus laevis. Fry AM;Descombes P;Twomey C;Bacchieri R;Nigg EA
  9. J Cell Sci v.110 Nuclear envelope breahdown in mammalian cells involves stepwise lamina disassembly and microtubule-drive deformation of thw unclear membrane. Georgatos SD;Pyrpasopoulou A;Theodoropoulos PA
  10. Cell v.81 Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles. Glover DM;Leibowitz MH;McLean DA;Parry H https://doi.org/10.1016/0092-8674(95)90374-7
  11. Nucleic Acids Res v.29 KH domain:one motif, two folds. Grishin NV https://doi.org/10.1093/nar/29.3.638
  12. Mol Cells v.12 Yeast ribosomal protein S3 has an endonuclease activity on AP DNA. Jung SO;Lee JY;Kim J
  13. J Mol BIOL v.298 Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm. Kim J;Hajm B;Kim Y;Choi M;Jang SK
  14. Biochem Biophys Rec Commun v.290 Nek2 localizes to multiple sites in mitotic cells, suggesting its involvement in miltiple cellar functions during the cell cycle. Kim Y;Choi J;Jeong Y;Wolgemuth DJ;Rhee K https://doi.org/10.1006/bbrc.2001.6212
  15. EMBO J v.17 A noble direct interaction of endoplasmic reticulum with microtubules. Klopfenstein DR;Kappeler F;Hauri HP https://doi.org/10.1093/emboj/17.21.6168
  16. J Biol Chem v.275 Regulation of X11L-depdendent amyloid precursor protein metabolism by XB51, a noble X11L-binding protein. Lee D;Tomita S;Kirino Y;Suzuki T https://doi.org/10.1074/jbc.C000302200
  17. Curr Opin Struct Biol v.10 EF-hand calcium-binding proteins. Lewit-Bentley A;Rety S https://doi.org/10.1016/S0959-440X(00)00142-1
  18. Proc Natl Acad Sci USA v.97 Peripheral Golgi protein GRASP65 is a target of mitotic polo-like kinase(Plk)and Cdc2. Lin CY;Madsen ML;Yarm FR;Jang YJ;Liu X;Erikson RL https://doi.org/10.1073/pnas.220423497
  19. Mol Cell Biol v.19 Overproduction of human Myt1 kinase induces a G2 cell cycle delay by interfering with the intracellular trafficking of Cdc2-cyclin B1 complexes. Liu F;Rothblum-Oviatt C;Ryan CE;Piwnica-Worms H
  20. J Cell Sci v.114 SH3 domains:complexity in moderation. Mayer BJ
  21. J Cell Biol v.151 The centrosomal protein C-Nap1 is required for cell cyclepregulated centrosome cohesion. Mayor T;Stierhof YD;Tanaka K;Fry AM;Nigg EA https://doi.org/10.1083/jcb.151.4.837
  22. Nat Cell Biol v.3 Mitosis:a histroy of division. Mitchison TJ;Salmon ED https://doi.org/10.1038/35050656
  23. Mol Endocrinol v.15 Cloning and characterization of gonado-tropin-inducible ovarian transcription fators(GIOT1 and -2)that are noble members of the (Cys)(2)-(His)(2)-type zinc finger protein family. Mizutani T;Yamada K;Tazawa T;Okada T;Minegishi T;Miyamoto K https://doi.org/10.1210/me.15.10.1693
  24. Curr Opin Cell Biol v.10 Polo-like kinases:positive regulators of cell division from start to finish. Nigg EA https://doi.org/10.1016/S0955-0674(98)80121-X
  25. Development v.124 The NIMA-related kinase 2,Nek2, is expressed in specific stages of the meiotic cell cycle and associates with meiotic chromosomes. Rhee K;Wolgemuth DJ
  26. J Cell Sci v.104 Characterization of a noble 63 kDa membrane protein, Implications for the organization of the ER-to-Golgi pathway. Schweizer A;Ericsson M;Bachi T;Griffiths G;Hauri HP
  27. Cell Growth Differ v.4 Identification of 21 noble human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans. Schultz SJ;Nigg EA
  28. EMBO J v.19 Nek2B,a noble maternal form of Nek2 kinase, is essential for the assembly or maintenance of centrosomes in early Xenopus embryos. Uto K;Sagata N https://doi.org/10.1093/emboj/19.8.1816