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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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The Anti-proliferative Gene TIS21 Is Involved in Osteoclast Differentiation

  • Lee, Soo-Woong (National Research Laboratory for Bone Metabolism, Research Center for Proteineous Materials, and School of Dentistry) ;
  • Kwak, Han-Bok (National Research Laboratory for Bone Metabolism, Research Center for Proteineous Materials, and School of Dentistry) ;
  • Lee, Hong-Chan (National Research Laboratory for Bone Metabolism, Research Center for Proteineous Materials, and School of Dentistry) ;
  • Lee, Seung-Ku (National Research Laboratory for Bone Metabolism, Research Center for Proteineous Materials, and School of Dentistry) ;
  • Kim, Hong-Hee (National Research Laboratory for Bone Metabolism, Research Center for Proteineous Materials, and School of Dentistry) ;
  • Lee, Zang-Hee (National Research Laboratory for Bone Metabolism, Research Center for Proteineous Materials, and School of Dentistry)
  • Published : 2002.11.30
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Abstract

The remodeling process of bone is accompanied by complex changes in the expression levels of various genes. Several approaches have been employed to detect differentially-expressed genes in regard to osteoclast differentiation. In order to identify the genes that are involved in osteoclast differentiation, we used a cDNA-array-nylon membrane. Among 1,200 genes that showed ameasurable signal, 19 genes were chosen for further study. Eleven genes were up-regulated; eight genes were down-regulated. TIS21 was one of the up-regulated genes which were highly expressed in mature osteoclasts. To verify the cDNA microarray results, we carried out RT-PCR and real-time RT-PCR for the TIS21 gene. The TIS21 mRNA level was higher in differentiated-osteoclasts when compared to undifferentiated bone-marrow macrophages. Furthermore, the treatment with $1\;{\mu}M$ of a TIS21 antisense oligonucleotide reduced the formation of osteoclasts from the bone-marrow-precursor cells by ~30%. These results provide evidence for the potential role of TIS21 in the differentiation of osteoclasts.

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References

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