Asian-Australasian Journal of Animal Sciences

  • 제15권8호
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  • Pages.1198-1203
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  • 2002
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Expression of Stat5a Gene in Bovine Mammary Gland and its Effect on Proliferation of Mammary Epithelial Cells

  • Jeon, D.H. (Department of Genetic Engineering, Biotechnology Research Institute, College of Agriculture Chonnam National University) ;
  • Choi, Y.J. (School of Agricultural Biotechnology, Seoul National University) ;
  • Baik, M.G. (Department of Genetic Engineering, Biotechnology Research Institute, College of Agriculture Chonnam National University)
  • 투고 : 2001.07.26
  • 심사 : 2002.02.15
  • 발행 : 2002.08.01
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초록

To understand molecular mechanisms involved in bovine mammary gland growth, expression of stat5a gene was examined in bovine mammary tissues. We found that stat5a gene was highly induced at pregnant 7 and 8 months compared to virgin mammary tissues. To examine function of bovine stat5a in mammary epithelial cell proliferation, stat5a expression vector was transfected into mammary epithelial HC11 cells. Cell proliferation rate in stat5a gene-transfected cells was 26%, 95% and 85% higher at 24 h, 48 h and 72 h after seeding, respectively, compared to control vector-transfected cells. Results demonstrate that bovine stat5a enhances proliferation of mammary epithelial cells.

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참고문헌

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피인용 문헌

  1. Exogenous prolactin stimulates mammary development and alters expression of prolactin-related genes in prepubertal gilts1,2 vol.83, pp.4, 2005, https://doi.org/10.2527/2005.834825x