Journal of Gastric Cancer

The Korean Gastric Cancer Association (대한위암학회)
권호 표지

The Relationship of the Helicobacter pylori Virulence Factor Gene Subtype in Gastric Adenocarcinoma

위선암에서 Helicobacter pylori 독성인자와 유전자 아형의 관련성

  • Shin Jong Min (Department of Internal Medicine, Dong-A University Hospital) ;
  • Han Sang Young (Department of Internal Medicine, Dong-A University Hospital) ;
  • Keum Dong Joo (Department of Internal Medicine, Dong-A University Hospital) ;
  • Kim Kwang Jin (Department of Internal Medicine, Dong-A University Hospital) ;
  • Jee Sam Ryong (Department of Internal Medicine, Dong-A University Hospital) ;
  • Hong Gi Bong (Department of Internal Medicine, Dong-A University Hospital) ;
  • Lee Jong Hun (Department of Internal Medicine, Dong-A University Hospital) ;
  • Choi Seok Ryeol (Department of Internal Medicine, Dong-A University Hospital) ;
  • Shin Woo Won (Department of Internal Medicine, Dong-A University Hospital)
  • 신종민 (동아대학교 의과대학 내과학교실) ;
  • 한상영 (동아대학교 의과대학 내과학교실) ;
  • 금동주 (동아대학교 의과대학 내과학교실) ;
  • 김광진 (동아대학교 의과대학 내과학교실) ;
  • 지삼룡 (동아대학교 의과대학 내과학교실) ;
  • 홍기봉 (동아대학교 의과대학 내과학교실) ;
  • 이종훈 (동아대학교 의과대학 내과학교실) ;
  • 최석렬 (동아대학교 의과대학 내과학교실) ;
  • 신우원 (동아대학교 의과대학 내과학교실)
  • Published : 2002.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: The H. pylori cagA gene, vacA gene and iceA gene are considered to be important virurence factors that have been implicated in the development of gastric adenocarcinoma. It was reported that the presence of IS605 elements may be responsible for rearrangements and lead to partial or total deletions of the cag pathogenicity island (PAI) and the virulence of cag PAI may be changed. However, different results regarding the association between these virulence factors and clinical disease have been reported from different geographic regions. This study evaluated the relationship between H. pylori virulence factors such as cagA, vacA, iceA, IS605 and gastric adenocarcinoma. Materials and Methods: H. pylori isolates were obtained from 54 infected patients (24 cases of gastric adenocarcinoma, 30 cases of control). H. pylori isolates were identified by PCR with ureC gene and 16S rRNA. PCR was performed to examine cagA, vacA, iceA and IS605 genotypes. Results: Significant difference was found in the negative rates of cagA between gastric adenocarcinoma group and control ($62.5\%\;vs.\;33.3\%$ P=0.033). No significant difference was found in the prevalence of iceA, vacA between gastric adenocar cinoma and control. The genotype of cagA+ vacA s1-m1 iceA1 was predominant in H. pylori isolates irrespective of the clinical outcome. IS605 in PAI was not found in gastric adenocarcinoma gruop and control. The positive rates of IS605 in genome were $33.3\%$ in gastric adenocarcinoma group and $36.7\%$ in control (P>0.05). In gastric carcinoma, the positive rate of $cagA^{+}/IS605$ was lower than in control ($12.5\%\;vs\;40.0\%$, P=0.025) and the positive rate of cagA-/IS605 was higher than in control ($54.2\%\;vs\;23.3\%$, P=0.02). Conclusion: H. pylori virulence factors had not related significantly with gastric adenocarcinoma. Further study is needed to examine the specificity of H. pylori strains.

Keywords