THE JOURNAL OF KOREAN ORIENTAL ONCOLOGY (대한한방종양학회지)

Korean Association of Traditional Oncology (대한암한의학회)
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Experimental Studies on the Anti-tumor and the Immunomodulatory Effects of Jiaweicitaowan(加未慈桃丸)

가미자도환(加味慈桃丸)의 항암(抗癌) 및 면역증강효과(免疫增强效果)에 관한 부험적(實驗的) 연구(硏究)

  • Jeon, Young-Soo (Dept. of Oriental Pathology, College of Oriental Medicine, Kyung Hee University) ;
  • Shim, Bum-Sang (Dept. of Oriental Pathology, College of Oriental Medicine, Kyung Hee University) ;
  • Choi, Seung-Hoon (Dept. of Oriental Pathology, College of Oriental Medicine, Kyung Hee University) ;
  • Ahn, Kyoo-Sook (Dept. of Oriental Pathology, College of Oriental Medicine, Kyung Hee University)
  • 전영수 (경희대학교 한의과대학 병리학교실) ;
  • 심범상 (경희대학교 한의과대학 병리학교실) ;
  • 최승훈 (경희대학교 한의과대학 병리학교실) ;
  • 안규석 (경희대학교 한의과대학 병리학교실)
  • Published : 2002.12.30
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Abstract

This experimental study was carried out to evaluate the anti-tumor and the immunomodulatory effects of Jiaweicitaowan(加未慈桃丸) against cancer. The in vitro anti-tumor effects were evaluated by MTT assay. The cytotoxicity, extension of survival days, the effect of inhibition solid tumor which was induced sarcoma 180, and the changes of body weight were evaluated for in vivo effects of anti-tumor. To evaluate the immunomodulatory effects of Jiawei- citaowan(加未慈桃丸), delayed type hypersensitivity, hemagglutinin, hemolysin titers for humoral immune response, rosette forming cells for cell-mediated immune response, natural killer cell activity, proliferation of lymphocyte, productivty of Interleukin-2, and carbon clearance were measured with methotrexate treated mice. The results were as follows; 1. In the case of existence ability of tumor cell, IC50 had an anti-tumor ativity resulted 2.52mg/ml to SNU-C4. 0.41mg/ml to SNU-396, resulted to 0.09mg/mlSNU-1. 2. The groups of Jiaweicitaowan(加未慈桃丸) 10mg/ml, 20mg/kg had no body weight loss. reduction in intake of water and feed, so these had no toxicity. 3. In the case of the effect of extention of existence. the group of 20mg/kg Jiaweicitaowan(加未慈桃丸) extract treated group was showed 250% in ILS. 4. The effect of inhibition solid tumor was significantly decreased in both 10mg/kg, 20mg/kg of Jiaweicitaowan(加未慈桃丸) extract treated groups as compared with control group S. The groups of 10mg/kg, 20mg/kg Jiaweicitaowan(加未慈桃丸) had significant effect of body weight change compared to control group. 6. Delayed type hypersensitivity was not significant in both Jiaweicitaowan(加未慈桃丸) extract treated groups as compared with control group. 7. Hemagglutinin and Hemolysin titers were significantly increased by dose-dependent. so these results showed that the humoral immume respose was activated. 8. For the effect of rosette formimg cells was not significant in hoth Jiaweicitaowan(加未慈桃丸) extract treated groups as compared with control group. 9. Natural killer cell activity was significantly increased in both Jiaweicitaowan(加未慈桃丸) extract treated groups as compared with control group in the ratio of 100: 1, 50: 1 of effector and target cells, but in the ratio of 10:1, the Jiaweicitaowan(加未慈桃丸) extract treated groups were not significant. 10. The proliferation of lymphocyte and productivty of Interleukin-2 were significantly increased by dose-dependent in both 10mg/ kg, 20mg/ kg of Jiaweicitaowan(加未慈桃丸) extract treated groups as compared with control group. 11. In the phagocytic effect, the 20mg/kg of Jiaweicitaowan(加未慈桃丸) extract treated group showed the increasing effect with significance as compared with control group. According to the results, we can suggest that Jiaweicitaowan(加未慈桃丸) has the antitumor and the immunomodulatory effects.

Keywords

References

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