Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
권호 표지

Effects of Wolgukwhan Methanol Extract on Oxidative Liver Injury

월국환(越鞠丸) 메탄올 추출물이 산화적 간손상에 미치는 효과

  • Moon Jin-Young (Department of AM-Pointology, College of Oriental Medicine, Dongguk University)
  • 문진영 (동국대학교 한의과대학 경혈학교실)
  • Published : 2002.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: In traditional medicine, Wolgukwhan has been used for the treatment of digestive system disease, such as indigestion, brash, ructation, nausea and vomiting. This study was purposed to investigate the effects of Wolgukwhan methnol extract (WGWM) on oxidative liver cell injury. Methods: In vivo assay, we administerated acetaminophen(500mg/kg, i.p.) to starved mice 24hrs after pretreatment of WGWM for 6days. In the liver homogenates, lipid peroxide and glutathione(GSH) levels were measured. In addition, activities of hepatic enzyme, such as catalase, glutathione peroxidase(GPX), glutathione S-transferase(GST) were measured in the hepatic mitochondrial and cytosolic fractions. Results: In vivo administeration of WGWM showed effective inhibition of acetaminophen induced lipid peroxidation and elevations of glutathione level. The acetaminophen treatment resulted in a decrease of catalase, GPX and GST activities. By contrast, WGWM pretreatment increased compare to those of untreated groups. Conclusions: These results suggested that WGWM might protect against lipid peroxidation by free radicals, destruction of hepatic cell membranes.

Keywords

References

  1. J. Free Radic. Biol. Med. v.1 Pathological mechanisms in carbon tetra-chloride hepatotoxicity Brattin, W.J.;Glende, E.A.;Recknagel, R.O.
  2. Mol. Pharmacol. v.37 Autophagic degradation of protein generates a pool of ferriciron required for the killing of cultured hepatocytes by an oxidative stress Sakaida, I.;Marlene, E.;Farber, J.L.
  3. J. Free Radic. Biol. Med. v.12 Implication of free radical mechanism in ethanol induced cellular injury Nordmann, R.;Ribiere, C.;Rouach, H.
  4. Arch. Biochem. Biophys. v.269 ter-Butyl hydroperoxide kills cultured hepatocytes by peroxidizing membrane lipids Masaki, N.;Kyle, M.E.;Farber, J.L.
  5. Arch. Biochem. Biophys. v.270 Mitochondrial damage as a mechanism of cell injury in the killing of cultured hepatocytes by tert-butyl hydroperoxide Masaki, N.;Kyle, M.E.;Farber, J.L.
  6. J. Free Radic. Biol. Med. v.8 Free radicals, antioxidant enzymes and carcinogenesis Sun, Y.
  7. Lancet. v.344 Free radical, antioxidant and human disease Halliwell, B.
  8. Pharmacol. Ther. v.39 Role of vitamin A and $\beta$-carotene in radiation protection, relation to antioxidant properties Seifter, E.;Mendecki, J.;Holzman, S.;Kanofsky, J.D.;Freidenthal, E.;Davis, L.;Weinzwerg, J.
  9. Korean J. Toxicol. v.13 no.3 마우스에서 아세트아미노펜의 급성 간독성과 독물 동태학 서경원;류정상;김효정
  10. Biochemical Pharmacology v.28 Acute paracetamol intoxication of starved mice leads to lipid peroxidation in vivo Wended, A.;Feuerstein, S.;Kontz, K.H.
  11. The Journal of Pharmacology and Experimental Therapeutics v.220 Protective effect of propylthiouracill indepentent of its hypothyroid effect in acetaminophen toxicity in the rat Raheja, K.L.;Linscheer, W.G.;Cho, C.D.;Mahany, D.
  12. The Journal of Pharmacology and Experimental Therapeutics v.220 Levels of acetaminophen and its metabolites in mouse tissues after a toxic does Fischer, L.J.;Green, M.D.;Harman, A.W.
  13. 단계의집 주진형
  14. 한방임상처방학 이상인;박선동
  15. 월국환 및 월국환가미방이 항스트레스 효과에 관한 실험적 연구 구병수
  16. 월국환이 비만생쥐의 난소반응과 임신에 미치는 영향 양성우
  17. 월국환과 칠기탕이 백서의 실험적 위궤양에 미치는 영향 문상원
  18. Analytical Biochem. v.95 Assay for lipid peroxides animal tissues by thiobarbituric acid reaction Ohkawa, H.;Ohishi, N.;Yagi, K.
  19. Anal. Chem. v.25 Extimation of total protein-bound and nonprotein-bound sulfhydryl groups in tissue with Ellman's reagent Sedlak, T.;Lindsay, R.H.
  20. Proteins, Nucleic Acid and Enzyme v.33 Critical review on the determination of glutathione in biological perparation Higach, T.
  21. Methods of enzymatic acalysis(2nd edition) Aebi, H.;Hans Ulrich Begmeyer(ed.)
  22. Lab. Clin. Med. v.70 Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase Paglia, D.E.;Valentine, W.N.
  23. Biochem. Biophysi. Res. Comm. v.71 Glutathione peroxidase activity in selenium-dificient rat liver Lawrence, R.A.;Burk, R.F.
  24. J. Biochem. v.249 no.22 Glutathione-S-transferase : the first enzymatic step mercapturic acid formation Habig, W.H.;Pabst, M.J.;Jabby, W.B.
  25. Anal. Biochem. v.150 Measurement of protein using bicichoninic acid Smith, P.K.;Krohn, R.I.;Hermanson, G.T.;Mallia, A.K.;Gartner, F.H.;Provenzano, M.D.;Fujimoto, E.K.;Goeke, B.J.;Klenk, D.C.