Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제28권3호
  • /
  • Pages.216-225
  • /
  • 2002
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  • 2234-7550(pISSN)
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  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
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히알루론산이 골 형성에 미치는 영향에 관한 실험적 연구

THE EFFECT OF HYALURONIC ACID ON MOUSE CALVARIA PRE-OSTEOBLASTS OSTEOGENESIS IN VITRO

  • 조용민 (원광대학교 치과대학 구강악안면외과학교실) ;
  • 민승기 (원광대학교 치과대학 구강악안면외과학교실) ;
  • 김수남 (원광대학교 치과대학 구강악안면외과학교실) ;
  • 유용욱 (원광대학교 치과대학 구강생화학교실)
  • Cho, Yong-Min (Department of Oral & Maxillofacial Surgery, School of Dentistry, Won-kwang University) ;
  • Min, Seung-Ki (Department of Oral & Maxillofacial Surgery, School of Dentistry, Won-kwang University) ;
  • Kim, Soo-Nam (Department of Oral & Maxillofacial Surgery, School of Dentistry, Won-kwang University) ;
  • You, Yong-Ouk (Dept. of Oral Biochemistry, School of Dentistry, Won-kwang University)
  • 발행 : 2002.06.30
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초록

Hyaluronic acid (HA) is an almost essential component of extracellular matrices. Early in embryogenesis mesenchymal cells migrate, proliferate and differentiate, in part, because of the influence of HA. Since the features of embryogenesis are revisited during wound repair, including bone fracture repair, this study was initiated to evaluate whether HA has an effect on calcification and bone formation in an in vitro system of osteogenesis. Mouse calvaria Pre-osteoblast (MC3T3-E1) cells were cultured in ${\alpha}-MEM$ medium with microorganism-derivative hyaluronic acid that was produced by Strep. zooepidemicus which of molecular weight was 3 million units. The dosages were categorized in each 0.5, 1.0 and 2.0 mg/ml concentration experimental groups. After 2 and 4 days cultures in expeirmental and control groups, the tendency of cell proliferation, MTT assay, protein synthesis ability, collagen synthesis and alkaline phosphatase activity were analysed and bone nodule formation capacity were measured with Alizarin Red S stain after 29 days cultures. The cell proliferation was increased in time, especially the group of 0.5 and 1.0 mg/ml concentration of HA were showed prominent cell proliferation. After 2 and 4 days culture, experimental groups in general were greater cell activity in MTT assay. The protein synthesis was increased in all experimental groups compared to control group, especially most prominent in 1.0 mg/ml concentration group. The collagen synthesis capacity were increased in HA experimental groups, especially prominent in 1.0 mg/ml group and the activity of alkaline phosphatase were increased, especially also prominent in 1.0 mg/ml group, compared to control group. Above these, the activity of mouse carvarial pre-osteoblast cells was showed greater bone osteogenesis activity in all applied HA experimental group, especially group of 1.0 mg/ml concentration of HA.

키워드

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