Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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PRODUCTION OF GM-CSF AND TGF-${\beta}1$ IN IRRADIATED HUMAN GINGIVAL FIBROBLASTS CULTURED WITH LIPOPOLYSACCHARIDE

Lipopolysaccharide로 자극시킨 방사선 조사 치은 섬유아 세포에서 granulocytemacrophage colony-stimulating factor와 transforming growth factor-${\beta}1$ 생성

  • Kim, Hong-Sik (Department of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University) ;
  • Lee, Seong-Geun (Department of Dentistry, College of Medicine, Ewha Womans University) ;
  • Kim, Kwang-Hyuk (Department of Microbiology, College of Medicine, Kosin University) ;
  • Kim, Uk-Kyu (Department of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University) ;
  • Kim, Jong-Ryoul (Department of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University) ;
  • Chung, In-Kyo (Department of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University) ;
  • Yang, Dong-Kyu (Department of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University)
  • 김홍식 (부산대학교 치과대학 구강악안면외과학교실) ;
  • 이성근 (이화여자대학교 의과대학 치과학교실) ;
  • 김광혁 (고신대학교 의학대학 미생물학교실) ;
  • 김욱규 (부산대학교 치과대학 구강악안면외과학교실) ;
  • 김종렬 (부산대학교 치과대학 구강악안면외과학교실) ;
  • 정인교 (부산대학교 치과대학 구강악안면외과학교실) ;
  • 양동규 (부산대학교 치과대학 구강악안면외과학교실)
  • Published : 2002.06.30
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Abstract

Purpose: Irradiation in the oral cancer patients causes early and late complications such as intraoral mucositis and fibrosis, with a various expression of GM-CSF and TGF-${\beta}1$. The purpose of this study was to investigate the production of GM-CSF and TGF-${\beta}1$ by the irradiated human gingival fibroblasts cultivated with lipopolysaccharide. Materials and Methods: Irradiated (total dose, 60 Gy) human gingival fibroblasts were incubated with LPS. Culture supernatants that were collected at 24, 48, and 72 hours were assessed for GM-CSF and TGF-${\beta}1$ by enzyme-linked immunosorbent assay. Results: 1. GM-CSF production in nomal gingival fibroblasts was increased with incubation time, but decreased with incubation time in irradiated gingival fibroblasts. GM-CSF production in both normal and irradiated gingival fibroblasts induced with LPS was higher than the control. 2. TGF-${\beta}1$ production in normal gingival fibroblasts was decreased after 24 hours, but, it was increased until 48 hours in irradiated gingival fibroblasts. TGF-${\beta}1$ production in normal gingival fibroblasts exposed with LPS was higher than the control. Conversely, It was lower than the control in irradiated gingival fibroblasts exposed with LPS. Conclusion: This indicates that irradiation in gingival fibroblasts may play an important role in radiation-induced intraoral mucositis and fibrosis. However, LPS decreases the production of TGF-${\beta}1$ in the irradiated gingival fibroblasts.

Keywords

References

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