DOI QR코드

DOI QR Code

Differential role of endothelium in hawthorn fruit extract-induced relaxation of rat cerebral, coronary, carotid, and aorta

  • Chan, Hoi Yun (Department of Physiology, Chinese University of Hong Kong) ;
  • Chen, Zhen-Yu (Department of Biochemistry, Chinese University of Hong Kong) ;
  • Yao, Xiaoqiang (Department of Physiology, Chinese University of Hong Kong) ;
  • Lau, Chi-Wai (Department of Physiology, Chinese University of Hong Kong) ;
  • Zhang, ZeSeng (Department of Biochemistry, Chinese University of Hong Kong) ;
  • Ho, Walter Kwok Keung (Department of Biochemistry, Chinese University of Hong Kong) ;
  • Huang, Yu (Department of Physiology, Chinese University of Hong Kong)
  • 발행 : 2002.08.30

초록

The present study was aimed to examine the role of endothelium in the relaxant effect of hawthorn fruit extract of Crataegus pinnatifida in four different types of rat arteries, posterior cerebral communicating artery, right descending coronary artery, common carotid artery, and aorta. In $9,11-dideoxy-11{\alpha}$, $9{\alpha}-epoxy-methanoprostaglandin$ $F_{2{\alpha}}$ (U46619)-preconstricted arterial rings except for aorta, the extract produced endothelium-independent relaxations with similar potency. This relaxation was unaffected by pretreatment with $100\;{\mu}M\;N^G-nitro-L-arginine$ methylester (L-NAME, the nitric oxide synthase inhibitor), $3\;{\mu}M$ 1H-[l,2,4]oxadiazolo$[4,2-{\alpha}]$quinoxalin-1-one (ODQ, the guanylate cyclase inhibitor), or $10\;{\mu}M$ indomethacin (the cyclooxygenase inhibitor). Putative $K^+$ channel blockers (charybdotoxin plus apamin or glibenclamide) did not affect the extract-induced relaxation in cerebral or coronary artery rings. In contrast, in rat aortic rings the extract produced significantly smaller relaxant response in endothelium-denuded rings than that in endothelium-intact rings. Pretreatment with L-NAME or ODQ abolished the extractinduced endothelium-dependent aortic relaxation, whilst indomethacin $(3\;{\mu}M)$ had no effect. The present results indicate that hawthorn fruit extract possesses a vasorelaxing effect in cerebral, coronary and carotid arteries and this effect is independent of the presence of a functional endothelium. However, the extract-induced endothelium-dependent relaxation in rat aorta was mediated through endothelial nitric oxide and cyclic GMP-dependent mechanisms, suggesting that active components in the extract may act on endothelium to stimulate release of nitric oxide in large conduit arteries of the rats.

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참고문헌

  1. Bahorun T, Gressier B, Trotin F, Brunet C, Dine T, Luyckx M, Vasseur J, Cazin M, Cazin JC, Pinkas M. (1996) Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittelforschung 46, 1086-1089.
  2. Bahorun T, Trotin F, Pommery J, Vasseur J, Pinkas M. (1994) Antioxidant activities of Crataegus monogyna extracts. Planta Med. 60, 323-328. https://doi.org/10.1055/s-2006-959493
  3. Chen ZY, Zhang ZS, Kwan KY, Zhu M, Ho WKK, Huang Y. (1998) Endothelium-dependent relaxation induced by hawthorn extract in rat mesenteric artery. Life Sci. 63, 1983-1991. https://doi.org/10.1016/S0024-3205(98)00476-7
  4. Daut J, Klieber HG, Cyrys S, Noack T. (1994) $K_{ATP}$ channels and basal coronary vascular tone. Cardiovasc. Res. 28, 811-817. https://doi.org/10.1093/cvr/28.6.811
  5. Edwards G, Dora KA, Gardener MJ, Garland CJ, Weston AH. (1998) $K^{+}$ is an endothelium-derived hyperpolarizing factor in rat arteries. Nature 396, 269-272. https://doi.org/10.1038/24388
  6. Holubarsch CJ, Colucci WS, Meinertz T, Gaus W, Tendera M. (2000) Survival and prognosis: investigation of Crataegus extract WS 1442 in congestive heart failure (SPICE)-rationale, study design and study protocol. Eur. J. Heart Fail. 2, 431-437. https://doi.org/10.1016/S1388-9842(00)00109-4
  7. Huang KC. (1999) Anti-hypercholesterolemic herbs. In: The Pharmacology of Chinese Herbs, 2nd edn, p. 117-20, CRC Press LLC, Florida.
  8. Kim SH, Kang KW, Kim KW, Kim ND. (2000) Procyanidins in crataegus extract evoke endothelium-dependent vasorelaxation in rat aorta. Life Sci. 67, 121-131. https://doi.org/10.1016/S0024-3205(00)00608-1
  9. Mavers WH, Hensel H. (1974) Changes in local myocardial blood circulation following oral administration of a Crataegus extract in non-narcotized dogs. Arzneimittelforschung 24, 783-785.
  10. Nasa Y, Hashizume H, Hoque AN, Abiko Y. (1993) Protective effect of crataegus extract on the cardiac mechanical dysfunction in isolated perfused working rat heart. Arzneimittelforschung 43, 945-949.
  11. Standen NB, Quayle JM, Davies NW, Brayden JE, Huang Y, Nelson MT. (1989) Hyperpolarizing vasodilators activate ATP-sensitive $K^{+}$ channels in arterial smooth muscle. Science 245, 177-180. https://doi.org/10.1126/science.2501869
  12. Weiss RF, Fintelmann V. (2000) Herbal Medicine, Georg Thieme Verlag, New York.
  13. Zhang ZS, Chang Q, Zhu M, Huang Y, Ho WKK, Chen ZY. (2001) Characterization of antioxidants present in hawthorn fruits. J. Nutr. Biochem. 12, 144-152. https://doi.org/10.1016/S0955-2863(00)00137-6
  14. Zhang ZS, Ho WKK, Huang Y, James AE, Lam LW, Chen ZY. (2002) Hawthorn fruit is hypolipidemic in rabbits fed a high cholesterol diet. J. Nutr. 132, 5-10. https://doi.org/10.1093/jn/132.1.5