Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Screening of Antioxidant in Fructus Mune (Prunus mune Sieb. et Zucc.) Extract

매실추출물의 항산화력 탐색

  • Park, Myung-Woo (Institute of Ag. Sci. and Tech., Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Kim, Mi-Ra (Institute of Ag. Sci. and Tech., Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Lim, Kye-Taek (Division of Biodefensive Substances, Institute of Biotechnology Chonnam National University) ;
  • Park, Seung-Taeck (School of Medicine, Wonkwang University) ;
  • Shim, Jae-Han (Institute of Ag. Sci. and Tech., Division of Applied Bioscience and Biotechnology, Chonnam National University)
  • 박명우 (전남대학교 농업과학기술연구소, 응용생물공학부) ;
  • 김미라 (전남대학교 농업과학기술연구소, 응용생물공학부) ;
  • 임계택 (전남대학교 생물공학연구소 생활성물질부) ;
  • 박승택 (원광대학교 의과대학 해부학교실) ;
  • 심재한 (전남대학교 농업과학기술연구소, 응용생물공학부)
  • Published : 2002.05.31
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Abstract

Antioxidative activities of ethanol extracts of fruits from three cultivars of Prunus mune with different harvest times were determined. Extracts of P. mune cutivar Okyoung showed the strongest activity at 97.5%(compared to BHT) using DPPH or thiocyanate method, although no statistically significant differences were observed among the cultivars. Antioxidative activities of the extracts measured with glutathione-S-transferase method was over 70% of that of BHT. Survival rates of cells in mouse spinal cord culture challenged by superoxide anion were 89, 79, and 62% at 40 mg/ml of Okyoung, Namgo, and Kochunme extracts, respectively, whereas 90% at 6mM glutathion.

매실(Prunus mune Sieb. et Zucc.)의 품종간, 그리고 수확기에 따라 매실추출물의 항산화력을 알아보기 위하여 본 실험을 수행하였다. 먼저 항산화력은 1,1-diphenyl-2-picryl hydrazyl(DPPH), thiocyanate, glutathione-Stransferase와 같은 화학적 방법과 생물학적 방법으로 mouse의 spinal cord culture system에 superoxide anion을 발생시켜 측정했다. 일반적으로 매실 에탄을 추출물은 항산화력을 갖고 있었으며 DPPH, thiocyanate방법에 있어서 butylated hydroxy toluene(BHT)와 비교하였을 때 옥영이 다른 품종보다 97.5%로 제일 높았으나 품종간에 유의성은 없었다. Glutathione-S-transferas방법으로 butylated hydroxy toluene(BHT)와 매실추출물의 항산화력을 비교해본결과 70% 이상의 강한 항산화력을 보여주었다. 생쥐의 척수신경세포 배양시 세포생존율은 옥영, 남고, 고전매추출물을 40 mg처리했을 때 각각 89, 79그리고 62%였고, 6린M의 glutathion을 처리했을때는 90%였다.

Keywords

References

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