Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Shoot Regeneration from the Leaf Explants of Tetragonia tetragonoides $O.\;K_{UNTZE}$

번행초의 잎 절편으로부터 신초의 재분화

  • Hwang, Sung-Jin (Department of Food and Biotechnology, Dongshin University) ;
  • Pyo, Byoung-Sik (Department of Food and Biotechnology, Dongshin University) ;
  • Hwang, Baik (Department of Biology, Chonnam National University)
  • 황성진 (동신대학교 식품생물공학과) ;
  • 표병식 (동신대학교 식품생물공학과) ;
  • 황백 (전남대학교 생물학과)
  • Published : 2002.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

A protocol has been developed for differentiation of adventitious shoots directly from leaf segments of Tetragonia tetragonoides O. Kuntze. Murashige and Skoog (MS) medium supplemented with 2 mg/L $N^6-benzyladenine$ (BA) and 0.5 mg/L ${\alpha}-naphthaleneacetic$ acid (NAA) supported the induction of adventitious shoots from leaf explants. Adventitious shoots were multiplied by subculturing on the double strength MS (2MS) medium supplemented with 0.5 mg/L NAA and 2 mg/L BA. Shoots were rooted on MS basal medium without any growth regulators.

번행초(Tetragonia tetragonoides)의 잎 절편으로부터 기내(in vitro) 증식을 유도 하였다. 2 mg/L BA와 0.5 mg/L NAA가 조합 처리된 MS 기본배지에서 잎 절편으로부터 직접 기관형성 과정을 통하여 부정아가 형성 되었다. 유기한 부정아는 절취하여 2 mg/L NAA와 0.5 mg/L BA이 첨가된 MS배지로 옮겨 유묘의 대량 증식을 시도하였으며, 배양 3주 후 신초의 지속적인 성장을 위해 무기염의 농도가 두배로 증가된 2MS배지로 옮겨 배양 하였다. 증식된 신초로부터 발근은 식물성장조절물질이 첨가되지 않은 1/2MS와 MS배지 모두에서 이루어 졌다.

Keywords

References

  1. Boggetti B, Jasik J, Mantell S (1999) In vitro Multiplication of Anacardium occidentale L. using shoot node explants of glassraised plants. Plant Cell Rep. 18 : 456-461 https://doi.org/10.1007/s002990050603
  2. Cuena S, Amo-Marco JB (2000) In vitro Propagation of two spanish endemic species of Salvia trrough bud proliferation, In Vitro Cell Dev. Biol.-Plant. 36 : 225-229
  3. George L, Rao PS (1982) In vitro Multiplication of Carthamus tinctorius through tissue culture. Proc. Indian Acad. Sci. 99 :293-296
  4. Hazra S, Kulkarmi AV, Nalawade SM, Banerjee AK, Agrawal DC, Krishnamurthy KV (2000) Influence of explants, genotypes, and culture vessels on sprouting and proliferation of pre-existing meristems of cotton, In Vitro Cell Dev. BioL-Plant. 36 : 505-510 https://doi.org/10.1007/s11627-000-0090-7
  5. Lin HS, De-Jen MJ, Jacobson E (1997) Direct shoot regeneration from excised leaf explants of in vitro grown seedlings of Alstroemeria. Plant Cell Rep. 16 : 770-774 https://doi.org/10.1007/s002990050317
  6. Shivasta N, Rajani M (1999) Multiple shoot regeneration and tissue culture studies on Bocapa monnieri L. Plant Cell Rep. 18 : 919-923 https://doi.org/10.1007/s002990050684
  7. Stefaan P, Werbrouck O, Deberg PC (1994) Applied aspects of plant regeneration, In Plant Cell Culture: A practical approach. Dixson, R. A. ans R. Z. Gonzales ed. Oxford Univ. Press NY, pp. 127-145
  8. Sung CK, Kimura T, But PPH, Ji-Xian Guo, Han BH (1998) In International collation of traditional and folk medicin. World Sci. Press pp. 27-28
  9. Takayama S, Misawa M (1979) Differentiation in Lilium bulbscales grown in vitro. Physiol. Plant. 46 : 184-190 https://doi.org/10.1111/j.1399-3054.1979.tb06555.x
  10. Tejowath G, Anwar SY (1987) Plantlet regeneration from cotyledonary cultures of Cathamus tinctorius L. In Plant cell and tissue cultures of economically important plants. Reddy, G. M. ed. Hyderabead India, Osmania Univ. pp. 347-353
  11. Tripepi RR (1997) Advantitious shoot regeneration, In Biotechnology of omamental plants. Geneve. R. L., Preece, J. E., Merkle, S. A. ed. Wallingford, UK. pp. 45-71
  12. Tsay HS, Gau TG, Chen CC (1989) Rapid clonal propagation of Pinellia temata by tissue culture. Plant Cell rep. 8 : 450-454 https://doi.org/10.1007/BF00269046
  13. Turker AU, Camper ND, Gurel E (2001) In vitro Culture of common mullen (Verbascum thapsus L.). In Vitro Cell Dev. Biol.-Plant. 37 : 40-43 https://doi.org/10.1007/s11627-001-0008-z
  14. van-Alvorst AC, Koehorst HJJ, Bruinsma T, Dons JJM (1994) Improvement of advantitious shoot formation from carnation leaf explants. Plant Cell Tiss. Org. Cult. 37 : 87-90 https://doi.org/10.1007/BF00048123
  15. Wildi E, Schaffner W, Berger K (1998) In vitro Propagation of Petasites hybridus (Asteraceae) from leaf and petiole explants and inflorescence buds. Plant Cell Rep. 18 : 336-340 https://doi.org/10.1007/s002990050582