Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Preparation of Whelk Internal Organ Jeotgal with the Addition of Commercial Proteolytic Enzymes

상업용 단백질 가수분해 효소를 첨가한 골뱅이 내장 젓갈의 제조

  • Oh, Jeong-Hoon (Department of Food Science and Biotechnology, Division of Life Sciences, Kyungnam University) ;
  • Koo, Myung-O (Department of Food Science and Biotechnology, Division of Life Sciences, Kyungnam University) ;
  • Lee, Kyung-Eun (Department of Food Science and Biotechnology, Division of Life Sciences, Kyungnam University) ;
  • Lee, Seung-Cheol (Department of Food Science and Biotechnology, Division of Life Sciences, Kyungnam University)
  • 오정훈 (경남대학교 생명과학부 식품생물공학) ;
  • 구명오 (경남대학교 생명과학부 식품생물공학) ;
  • 이경은 (경남대학교 생명과학부 식품생물공학) ;
  • 이승철 (경남대학교 생명과학부 식품생물공학)
  • Published : 2002.08.01
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Abstract

For the utilization of the by-products of whelk processing, whelk internal organ with the addition of commercial proteolytic enzymes - Flavourzyme, Neutrase, Protease NP, Prozyme - were used to make jeotgal, Korean traditional salted and fermented seafood sauce. The products were prepared at salt concentration of 25% with enzyme contents 0.05 and 0.1%. The samples were stored at $10^{\circ}C$ and the chemical properties were evaluated for 6 months. The pH in all samples were decreased from near 6.8 in the beginning stage to 6.1-6.4 in the final stage of incubation. Amino nitrogen of jeotgal increased with enzyme concentration and showed maximum value, 646 mg%, at 0.1% of Flavourzyme. Total nitrogen content was increased till four months, but rapidly decreased after that. Protein degradations of whelk internal organ during maturation of jeotgals were investigated by SDS-PAGE. The patterns of degradation were different with added enzymes.

골뱅이의 가공 부산물인 내장의 효율적인 이용을 위해 골뱅이 내장을 마쇄한 후, 염을 25% 첨가하고 단백질 가수분해효소로서 상업용으로 많이 이용되는 Flavourzyme, Neutrase, Protease NP Prozyme을 각각 0.05, 0.1% 첨가하여 $10^{\circ}C$에서 6개월 동안 숙성하여 특성을 관찰하였다. 상업용 효소를 첨가한 모든 골뱅이 내장젓갈에서 숙성 30일까지 급격하게 감소하며, 숙성기간이 증가할수록 약간의 pH가 증가하였으며 숙성 150일에서 pH가 약간 감소하여 숙성기간 180일에서는 pH $6.1{\sim}6.4$까지 다시 증가하는 경향을 나타내었다. 특히, Flavourzyme 첨가구가 다른 효소 첨가구에 비해 pH 변화의 증감이 큰 편이며, Prozyme 첨가구는 이와는 달리, 점차적으로 pH의 감소경향을 나타내었다. 아미노 질소 변화는 효소 첨가량에 비례하여 증가하였으며, 최대 아미노 질소는 Flavourzyme 0.1% 첨가구가 646 mg%를 나타내었다. 총질소 함량은 숙성기간 120일까지는 계속적인 증가를 보였으며, 숙성 120일부터 180일까지 다시 급격하게 감소하는 경향을 보였으며, 효소에 따른 총질소 함량의 증감이 상이하게 관찰되었다. SDS-PAGE에 의한 골뱅이 내장젓갈의 분해결과는 모든 효소 첨가구에서 높은 염함량에도 불구하고 숙성기간의 증가에 따라 점차적으로 분해되는 것을 보였지만, 효소 첨가량에 따라서는 뚜렷한 변화가 관찰되지 않았다. 또한, 골뱅이 내장젓갈을 6개월간 숙성한 후, 시판되고 있는 2종류의 액젓과 색도를 비교하여 골뱅이 내장 젓갈이 L값이 2배 이상 높았고, a값과 b값은 거의 유사하게 측정되었다.

Keywords

References

  1. Lee, C.H. Fish fermentation technology in Korea, pp. 187-201.In: Fish Fermentation Technology. Lee, C.H., Steinkraus, K.H. and Reilly, P.J.A. (eds.). United Nations University Press, Seoul (1993)
  2. Hur, S.H. Critical review on the microbiological standardization of salt fermented fish product. J. Korean Soc. Food Sci. Nutr. 25:885-891 (1996)
  3. Lee, C.H., Lee, E.H., Lim, M.H., Kim, S.H., Chai, S.K., Lee, K.W. and Koh, K.H. Fermented Fish Products in Korea. pp. 7-8.Yulim Publishing Co., Seoul (1987)
  4. Lee, K.H. Microbiological and enzymological studies on the fla-vor components of sea food pickles. Agric. Chem. Biotechnol.11: 1-27(1969)
  5. Suh, M.J. Changes in protease and formomtrogen of salted fish and shellfish. Korean. J. Nutr. 6: 123-134 (1973)
  6. Kim, Y.M. Processing technique and quality control of fermented seafood. Bull. Food Technol. 9: 65-86 (1996)
  7. Cha, Y.J., Lee, E.H., Lee, K.H. and Chang, D.S. Characterization of the strong proteolytic bacteria isolated from low salt fermented anchovy and of protease produced by that strain. Bull. Korean Fish. Soc.21:71-79(1988)
  8. Cha, Y.J. and Lee, E.H. Studies on the processing of rapid fer-mented anchovy prepared with low salt contents by adapted microorganism. (1) Biochemical characterization of proteolytic bacteria and their extracellular protease isolated from fermented fish paste. Bull. Korean Fish. Soc. 22: 363-369 (1989)
  9. Suh, H.K. and Yoon, S.S. A study on the regional characteristics of Korean chotkal - The kinds and materials of chotkal. Korean J.Dietary Culture 2: 45-54 (1987)
  10. Kim, Y.M., Lee, Y.C., Koo, J.G. and Kim, D.S. Preparation of the hydrolysate using crab byproduct after water extraction. Bull. Korean Fish. Soc. 23: 77-86 (1990)
  11. Lee, Y.C., Kim, D.S., Kim, Y.D. and Kim, Y.M. Preparation of oyster (Crassostrea gigas) and sea mussel (Mytilus coruscus)hydrolysates using commercial protease. Korean J. Food Sci.Technol. 22: 234-240 (1990)
  12. Kang, H.I., Kang, T.J., Bae, T.J. and Kim, H.J. Processing of fer-mented squeezed-type pen shell by-product by proteolytic enzyme. Bull. Korean Fish. Soc. 27: 509-514 (1994)
  13. Kim, S.K., Park, P.J. and Lim, G.H. Preparation of sauce from enzymatic hydrolysates of cod frame protein. J. Korean Soc. Food Sci. Nutr. 29: 635-641 (2000)
  14. Oh, K.S. Processings of intermediate flavoring substance from low-utilized long finned squid. J. Korean Soc. Food Sci. Nutr. 29:663-668 (2000)
  15. Oh, J.H., Lee, K.E., Kim, J.M. and Lee, S.C. Characteristics of whelk internal organ jeotgat with the addition of bromelain. Korean J. Food Sci. Technol. 33: 78-83 (2001)
  16. Oh, J.H., Lee, K.E., Kim, J.M. and Lee, S.C. Preparation and characteristics of whelk internal organ jeotgal with the addition of fruit juice. J. Korean Soc. Food Sci. Nutr. 30: 641-645 (2001)
  17. Ko, B.S., Hwang, Y.I. and Lee, S.C. Simple purification of bro-melain from pineapple. J. Food Sci. Nutr. 1: 106-110 (1996)
  18. Laemmli, U.K. Cleavage of structural proteins during the assem-bly of the head of bacteriophage T4. Nature 277: 751 (1970)
  19. AOAC. Official methods of analysis. 14th ed., Association of Official Analytical Chemists, Washington, DC, USA (1995)
  20. Lee, K.Y, Kim, H.S., Lee, H.G., Han, O. and Chang, U.J. Stud-ies on the prediction of the shelf-life of kochujang through thephysicochemical and sensory analyses during storage. J. Korean Soc. Food. Sci. Nutr. 26: 588-594 (1997)
  21. Kim, S.M., Jeong, I.H. and Cho, Y.J. The development of squid (Todarodes pacificus) sikhae in Kang-Nung district; 1. The Effects of fermentation temperatures and periods on the properties of squid sikhae. Bull. Korean Fish. Soc. 27: 215-222 (1994)
  22. Kim, S.M. The effects of sulfite salts on the shelf-life of low-salted Myungranjeot (Soused Roe of Alaska Pollack). Korean J. Food Sci. Technol. 28: 940-946 (1996)
  23. Imm, J.Y. and Lee, C.M. Production of seafood flavor from red hake (Urophycis chuss) by enzymatic hydrolysis. J. Agric. Food Chem. 46: 2360-2366 (1999)
  24. Jo, J.H., Oh, S.W., Kim, Y.M. and Chung, D.H. Conditions of water activity of raw material and adding levels of papain and glucose for processing fermented squid with low salt concentra-tions. Korean J. Food Sci. Technol. 24: 62-68 (1998)
  25. Lee, Y.Z., Simpson, B.K. and Haard, N.F. Supplementation of squid fermentation with proteolytic enzymes. J. Food Biochem. 6:127(1982) https://doi.org/10.1111/j.1745-4514.1982.tb00301.x
  26. You, B.J. and Chang, M.H. Processing of low salt fermentedsauce of shellfish with citric acid pretreatment. Korean J. Food Sci. Technol. 24: 541-546 (1992)
  27. Lee, E.H., Cha, Y.J. and Lee, J.S. Studies on the processing of low salt fermented sea foods: 1. Processing conditions of low salt fermented sardine. Bull. Korean Fish. Soc. 16: 133-139 (1983)
  28. Youn, J.E. and Yang, R. Studies on the aging of beef at addingthe proteolytic enzyme. IV. Studies on the tenderness effect ofbeef by papain treatment. Korean J. Food Sci. Technol. 6: 163-168 (1974)
  29. Lee, D.S., Suh, E.S. and Lee, K.H. Processing and packaging of anchovy sauce. J. Korean Soc. Food Sci. Nutr. 25: 1087-1093(1996)