Korean Journal of Oriental Medicine (한국한의학연구원논문집)
- Volume 8 Issue 2 Serial No. 9
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- Pages.75-84
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- 2002
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- 1229-3377(pISSN)
Gene Expression Profile in Epididymal Adipose Tissue from High-fat Diet Fed Mice
고지방식이를 섭취한 mouse에서의 유전자군의 발현양상 변화
- Cha, Min-Ho (Korea Institute of Oriental Medicine R&D) ;
- Kim, Kyung-Seon (Korea Institute of Oriental Medicine R&D) ;
- Sim, Woong-Seop (Department of lifescience, Korea Univ.) ;
- Yoon, Yoo-Sik (Korea Institute of Oriental Medicine R&D)
- Published : 2002.12.31
Abstract
Obesity can be defined as a metabolic disease due to a increased state of fat tissue caused by an imbalance of calorie intake and use. To define genes that affected by different nutrient, we study gene expression from mice which were fed different nutrient. Epididymal and retro-peritineal adipose tissue were increase in high fat diet feeding mice compared with control, but liver and spleen were not. In serum, total cholesterol were differently increase in high fat diet feeding mice but total triglyceride and free fatty acid were not. That was maybe result of energy balance regulation in vivo system. aP2, PPART2 and FAS genes that were increased during adipogenesis were inclosed in high fat diet fed mice compared with control. In microarray assay, 1.4% of total genes were affected in epididymal adipose tissue by different nutrient. 1.1% of total genes were decreased down 0.5 fold and 0.3% were increased over 2 fold. These results indicated that many genes are affected in adipose tissue by nutrient.