Cholesterol Content and Formation of Cholesterol oxidative products(COPs) in Processed Squids

가열조건에 따른 오징어 Cholesterol과 Cholesterol oxidative products(COPs)의 함량변화에 관한 연구

  • Published : 2002.10.31

Abstract

The effect of cooking(boiling, steaming and baking) and drying on the cholesterol content and formation of oxidized cholesterols and acid value in squid(Japanese flying squid, Todarodes pacificus) was studied. Cholesterol content of live squid meat varied with the portion sampled. The data from spectophotometric assay ranged from 263.2 mg/100g(mantle) to 315.8 mg/100g(tentacle). The cholesterol levels found for squid samples analyzed by gas chromatography(GC) were lower by 7% of total cholesterol for live squid meat and 24% for processed meat than those results by spectrophotometric assay. Cooking resulted some decrease in the initial total cholesterol content of raw meat from 10%(boiling for 5 min.) to 25%(steaming for 5 min.). The amounts of cholesterol remaining after baking were 68% for microwave oven samples and 64% for convection oven samples. Drying of raw tissue caused the greater reduction in cholesterol content than cooking but brought about no significant difference in samples stored for 6 weeks at $4^{\circ}C\;and\;20^{\circ}C$. Raw squid meats contained essentially no oxidized cholesterols, while the 22-hydroxychoesterol was detected in frozen meats. The additional oxidized cholesterols as cholestane-triol was indentified with 22-hydroxycholesterol in cooked samples. Sun dried meat stored at $4^{\circ}C\;and\;20^{\circ}C$ for 6 weeks had the three kinds of oxidized cholesterols such as 22-hydroxycholesterol, cholesta-3,5-dien-7-one and cholestane-triol. For the boiled and steamed squids, 10% higher acid value and 5% higher acid value respectively were observed but oven cooked samples resulted in a 50% higher acid value than raw samples. Squids had a 45% higher acid value than raw one during sundrying and presrevation at $20^{\circ}C$ but there was not a severe difference of acid value between $4^{\circ}C\;and\;20^{\circ}C$ stored samples.

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References

  1. Fincchiaro ET, Richardson T, Steil Oxides in Foodstuffs, A Review J Food, 46:917, 1983
  2. 管野道廣,泉勝己, フレステロ-ル, p29, 新光出版社,1972
  3. 須山三千三, 鴻巢 章二, 兵部 基次, 與田 行雄, 脂質,イカの利用,p62, 恒星社厚生閣, 東京, 1980
  4. Smith LL, Cholesterol Autoxidation, p 125, Plenum Press, New York., 1981
  5. Peng SK, Philip T, Tayor LB, Mikkelson B, Cytotoxidty of oxidation derivative of cholesterol on cultured aortic smooth muscle cells and their effect on cholesterol biosynthesis. Am. J. Clin. Nutri., 32:1033, 1979
  6. Bligh EC, Dyer WJ, A ripid method of total lipid extracted and purification, Can J Biochem Physiol, 37: 94, 1959
  7. Stroy E, Powrie WD, The method of cholesterol contents purification in spray-dried egg yolk, J Food Sci, 33:581, 1968 https://doi.org/10.1111/j.1365-2621.1968.tb09078.x
  8. Itoh T, Tamura T, Matsumoto T, Methylsterol Compositions of 19Vegetab1e Oils, J Am Oil. Chem, 50:300, 1973 https://doi.org/10.1007/BF02641360
  9. Krzynowek J, D'entremont DL, Murphy J, Proximate Composition and Fatty Acid and Cholesterol Content of Squid, Loligo peolei and illecebrosus, J Food Sci, 54:45, 1989 https://doi.org/10.1111/j.1365-2621.1989.tb08563.x
  10. Kovacs MIP, Anderson WE, Ackman RG, A simple method for determination of cholesterol and some plant sterol in fishery-based food products, J Food Sci, 1298:1305, 1979
  11. Osada K, Kodama T, Cui L, Yamada K, Sugano M, Levels and Formation of Oxidized Cholesterol in Processed Marine Foods, J Agric Food Chem, 41:1893, 1993 https://doi.org/10.1021/jf00035a016
  12. Krishnamoorthy RV, Verokataramiah A, Lakshmi GL, Biesiot P, Effects of Cooking and Frozen Sterage on the Cholesterol Content of Selected Shellfish, J Food Sci, 44: 314, 1979 https://doi.org/10.1111/j.1365-2621.1979.tb10077.x
  13. Park SW, Addis PB, Identification and quantitative estima-tion of oxidized cholesterol derivatives in heated tallow, J Agric Food Chem, 34:653, 1986 https://doi.org/10.1021/jf00070a016
  14. Turner FW, Payter WO, Mantie EJ, Use of acid value to measure rancidity in frozen squid, Food Technol, 8:326, 1994