The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Increase of Intracellular $Ca^{2+}$ Concentration Induced by Lysophosphatidylcholine in Murine Aortic Endothelial Cells

  • Zhu, Mei-Hong (Department of Physiology and Biophysics, Seoul National University College of Medicine) ;
  • Park, Sung-Jin (Department of Physiology and Biophysics, Seoul National University College of Medicine) ;
  • Kim, Hyun-Jin (Department of Physiology and Biophysics, Seoul National University College of Medicine) ;
  • Yang, Dong-Ki (Department of Physiology and Biophysics, Seoul National University College of Medicine) ;
  • Suh, Suk-Hyo (Department of Physiology, School of Medicine, Ewha Womans University) ;
  • So, In-Suk (Department of Physiology and Biophysics, Seoul National University College of Medicine) ;
  • Kim, Ki-Whan (Department of Physiology and Biophysics, Seoul National University College of Medicine)
  • Published : 2002.04.21
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Abstract

Effects of oxidized low-density lipoprotein (ox-LDL), $1-{\alpha}-stearoyl-lysophosphatidylcholine$ (LPC), on intracellular $Ca^{2+}$ concentration were examined in mouse endothelial cells by measuring intracellular $Ca^{2+}$ concentration $([Ca^{2+}]_i)$ with fura 2-AM and reverse transcription-polymerase chain reaction (RT-PCR). LPC increased $[Ca^{2+}]_i$ under the condition of 1.5 mM $[Ca^{2+}]_o$ but did not show any effect under the nominally $Ca^{2+}-free$ condition. Even after the store depletion with $30{\mu}M$ 2,5-di-tert- butylhydroquinone (BHQ) or $30{\mu}M$ ATP, LPC could still increase the $[Ca^{2+}]_i$ under the condition of 1.5 mM $[Ca^{2+}]_o.$ The time required to increase [$Ca{2+}$]i (about 1 minute) was longer than that for ATP-induced $[Ca^{2+}]_i$ increase $(10{\sim}30\;seconds).$ LPC-induced $[Ca^{2+}]_i$ increase was completely blocked by $1{\mu}M\;La^{3+}.$ Transient receptor potential channel(trpc) 4 mRNA was detected with RT-PCR. From these results, we suggest that LPC increased $[Ca^{2+}]_i$ via the increase of $Ca^{2+}$ influx through the $Ca^{2+}$ routes which exist in the plasma membrane.

Keywords

References

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