DOI QR코드

DOI QR Code

Production and Characterization of Monoclonal Antibodies to a Generic Hapten for-Class-Specific Determination of Organophosphorus Pesticides


Abstract

Monoclonal antibodies have been generated against a generic hapten, ο,ο-diethyl ο-(5-carboxy-2-fluorophenyl) phosphorothioate, for the determination of organophosphorus (OP) pesticides in a class-specific manner. In an indirect competitive enzyme-linked immunosorbent assay (ELISA) format, employing a heterologous coating antigen, these monoclonal antibodies showed desirable properties for use in the class-specific determination, i.e., broad specificity and high sensitivity. The IC50 values of four commonly used ο,ο-diethyl OP pesticides were fairly uniform ranging from 0.1 to 0.3 ㎛/mL. The IC50 values of three ο,ο-dimethyl derivatives were between 0.3 and 1.4 ㎛/mL. These values, together with the limits of detection (LOD), were better, in terms of the specificity and sensitivity, compared with the values obtained previously with polyclonal antibodies.

Keywords

References

  1. The Pesticide Manual: A World Compendium, 11th ed.; Tomlin, C.D. S., Ed.; The British Crop Protection Council: Farnham, Surrey,U.K., 1997.
  2. Racke, K.D. Rev. Environ. Contam. Toxicol. 1991, 131, 1-150.
  3. AOAC International. Official Methods of Analysis, 18th ed.;Arlington, VA, 1995; Section 970.52.
  4. Hennion, M.-C.; Barcelo, D. Anal. Chim. Acta 1998, 362, 3-34. https://doi.org/10.1016/S0003-2670(97)00608-9
  5. Banks, J. N.; Chaudry, M. Q.; Matthews, W. A.; Haverly, M.;Watkins, T.; Northway, B. J. Food Agric. Immunol. 1998, 10, 349-361. https://doi.org/10.1080/09540109809354998
  6. Johnson, J. C.; Van Emon, J. M.; Pullman, D. R.; Keeper, K. R. J.Agric. Food Chem. 1998, 46, 3116-3123. https://doi.org/10.1021/jf970191y
  7. Alcocer, M. J. C.; Dillon, P. P.; Manning, B. M.; Doyen, C.; Lee,H. A.; Daly, S. J. J. Agric. Food Chem. 2000, 48, 2228-2233. https://doi.org/10.1021/jf990691m
  8. McAdam, D. P.; Hill, A. S.; Beasley, H. L.; Skerritt, J. H. J. Agric.Food Chem. 1992, 40, 1466-1470. https://doi.org/10.1021/jf00020a035
  9. Galfre, G.; Miltstein, C. Methods Enzymol. 1981, 73, 3-46. https://doi.org/10.1016/0076-6879(81)73054-4
  10. Raab, G. M. Clin. Chem. 1983, 29, 1757-1761.

Cited by

  1. Development of a Class-specific Immunochromatographic Strip Test for the Rapid Detection of Organophosphorus Pesticides With a Thiophosphate Group vol.29, pp.4, 2010, https://doi.org/10.1089/hyb.2009.0111
  2. Destruction and Detection of Chemical Warfare Agents vol.111, pp.9, 2011, https://doi.org/10.1021/cr100193y
  3. A Novel Photoelectrochemical Sensor for the Organophosphorus Pesticide Dichlofenthion Based on Nanometer-Sized Titania Coupled with a Screen-Printed Electrode vol.83, pp.13, 2011, https://doi.org/10.1021/ac200706k
  4. Development of an Enzyme Linked Immunosorbent Assay and an Immunochromatographic Assay for Detection of Organophosphorus Pesticides in Different Agricultural Products vol.7, pp.12, 2012, https://doi.org/10.1371/journal.pone.0053099
  5. Multi-analyte enzyme-linked immunosorbent assay for organophosphorus pesticides and neonicotinoid insecticides using a bispecific monoclonal antibody vol.5, pp.6, 2013, https://doi.org/10.1039/c3ay26398c
  6. Immunoassay Development for the Class-Specific Assay for Types I and II Pyrethroid Insecticides in Water Samples vol.15, pp.1, 2010, https://doi.org/10.3390/molecules15010164
  7. Synthesis of three haptens for the class-specific immunoassay of O,O-dimethyl organophosphorus pesticides and effect of hapten heterology on immunoassay sensitivity vol.615, pp.2, 2002, https://doi.org/10.1016/j.aca.2008.03.050
  8. Effect of hapten structures on specific and sensitive enzyme-linked immunosorbent assays for N-methylcarbamate insecticide metolcarb vol.625, pp.1, 2002, https://doi.org/10.1016/j.aca.2008.07.003
  9. Production and characterization of a broad-specificity polyclonal antibody for O,O-diethyl organophosphorus pesticides and a quantitative structure–activity relationship study of antibody recogn vol.647, pp.1, 2009, https://doi.org/10.1016/j.aca.2009.05.025
  10. Application of computer-assisted molecular modeling for immunoassay of low molecular weight food contaminants: A review vol.647, pp.2, 2009, https://doi.org/10.1016/j.aca.2009.06.003
  11. Production of the class-specific antibody and development of direct competitive ELISA for multi-residue detection of organophosphorus pesticides vol.23, pp.2, 2012, https://doi.org/10.1080/09540105.2011.608120
  12. Immunochemical and molecular characteristics of monoclonal antibodies against organophosphorus pesticides and effect of hapten structures on immunoassay selectivity vol.26, pp.1, 2002, https://doi.org/10.1080/09540105.2013.873390