BMB Reports
- 제34권6호
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- Pages.584-589
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- 2001
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- 1976-670X(eISSN)
Characterization of the Small Cryptic Plasmid, pGD2, of Klebsiellia sp. KCL-2.
- Yoo, Ju-Soon (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
- Kim, Hae-Sun (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
- Chung, Soo-Yeol (Department of Food Science and Nutrition, Donju College) ;
- Lee, Young-Choon (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
- Cho, Young-Soo (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
- Choi, Yong-Lark (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University)
- 투고 : 2001.06.13
- 심사 : 2001.07.09
- 발행 : 2001.11.30
초록
One of the cryptic plasmids from the oil degrading bacterium Klebsiella sp. KCL-2, the small plasmid pGD2, has been identified and characterized. This plasmid has a size of 3.6 kb with unknown functions. We constructed the recombinant plasmid pMGD2. The nucleotide sequences of the plasmid were determined and two open reading frames were detected. ORF1 encodes a replication initiator protein (RepA), which has a high degree of homology with the protein of ColE2 plasmid. The product encoded by ORF2 showed a high similarity with the transposase protein of IS5. IS5 is 1195 by long and contains an inverted terminal repetition of 16 bp with one mismatch. Stem-loop structures in the 5'untranslated region of the repA suggest that a putative gene, incA, is located in a complementary strand to the leader region of the repA mRNA.