BMB Reports
- 제34권3호
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- Pages.230-236
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- 2001
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- 1976-670X(eISSN)
Acinetobacter calcoaceticus Glucose-1-phosphate Thymidylyltransferase: Cloning, Sequencing, and Expression in E.coli
- Eun, Suk-Ho (Programs in Biomateriais Science & Eng., Yonsei University) ;
- Kim, Dae-Jin (Programs in Biomateriais Science & Eng., Yonsei University) ;
- Kim, Yu-Sam (Department of Biochemistry, Yonsei University)
- 투고 : 2000.11.14
- 심사 : 2001.03.05
- 발행 : 2001.05.31
초록
dTDP-rhamnose is synthesized from dTTP and glucose-1-phosphate by four enzymatic steps in the gram-negative bacteria. By using a homologous PCR product, a gene cluster encoding four genes (rfbA, rfbB, rfbC, rfbD) involved in L-rhamnose biosynthesis by Acinetobacter calcoaceticus was isolated and sequenced. The four genes were clustered on the biosynthetic operon in the order of rfbB, D, A, C. A gene, rfbA, encoding glucose-l-phosphate thymidylyltransferase (RfbA), was cloned from A. calcoaceticus pathogenic and encapsulated in the gram-negative bacterium. This enzyme catalyzes the formation of dTDP-D-glucose From