BMB Reports
- 제34권2호
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- Pages.134-138
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- 2001
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- 1976-670X(eISSN)
The Effect of Sodium Chloride on the Serine-type Fibrinolytic Enzymes and the Thermostability of Extracellular Protease from Bacillus amyloliquefaciens DJ-4
- Choi, Nack-Shick (Protein Engineering Group, Korea Research Institute of Bioscience and Biotechnology) ;
- Kim, Seung-Ho (Protein Engineering Group, Korea Research Institute of Bioscience and Biotechnology)
- 투고 : 2000.11.10
- 심사 : 2000.12.18
- 발행 : 2001.03.31
초록
By adding sodium chloride (2.5%) into a Bacillus amyloliquefaciens DJ-4 culture broth, two serine-type fibrinolytic proteases with a molecular weight of 29 (subtilisin DJ-4) and 38-kDa were stimulated on the SDS-fibrin zymogram or inhibitor gels. B. amyloliquefaciens DJ-4 showed the highest proteolytic activity (5.52 plasmin NIH unit/ml) on the fibrin plate based on the molar ratio when cells were subjected to the 2.5% NaCl. Using a fibrin plate, the secreted protease from this strain in the presence of 5% NaCl showed that about 49% of the enzyme's activity remained after incubation at