The Korean Journal of Pain

The Korean Pain Society (대한통증학회)
권호 표지

Neurotoxicity of Sodium Salicylate on Spinal Sensory Neurons in Culture

배양 척수감각신경세포에 대한 살리실산 나트륨의 신경독성에 관한 연구

  • Lee, Kang-Chang (Department of Anesthesiology & Pain Clinic, School of Medicine Wonkwang University) ;
  • Choi, Yu-Sun (Department of Anesthesiology & Pain Clinic, School of Medicine Wonkwang University) ;
  • Park, Seung-Taeck (Department of Anatomy, School of Medicine, Wonkwang University)
  • 이강창 (원광대학교 의과대학 마취과학교실) ;
  • 최유선 (원광대학교 의과대학 마취과학교실) ;
  • 박승택 (원광대학교 의과대학 해부학교실)
  • Published : 2001.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Sodium salicylate (SS) is a nonsteroidal anti-inflammatory drug (NSAID) for the treatment of neuralgia or pain from rheumatoid arthritis. When abused or used in excess, SS can induce cytotoxicity. The present study examined whether SS has a neurotoxic effect. Methods: Cell viability was examined by MTT [3-(4,5-dimethylthiazol-2,5-dipheny ltetrazolium bromide] assay and Sulforhodamine (SRB) assay after cultivating dorsal root ganglion (DRG) neurons derived from neonatal mouse. These cells were treated with various concentrations of SS for 24 hours. In addition, the amount of protein synthesis against SS was measured in these cultures. Results: Cell viability (20, $40{\mu}g/ml$ SS) significantly decreased in a dose-dependent manner. Additionally, SS inhibited protein synthesis after the exposure of cultured mouse DRG neurons to $30{\mu}g/ml$ of SS for 24 hours. Conclusions: The present study suggests that SS is toxic in cultured DRG neurons derived from neonatal mouse by decreasing cell viability and the amount of protein synthesis.

Keywords