Korean Journal of Head & Neck Oncology (대한두경부종양학회지)

The Korean Society for Head and Neck Oncology (대한두경부종양학회)
권호 표지

Expression of Endothelial Nitric Oxide Synthase in Benign Nodular Hyperplasia and Papillary Carcinoma of Human Thyroid Gland

인간의 갑상선 결절성 과증식증과 유두상 암종에서의 Endothelial Nitric Oxide의 발현

  • Kim Young-Mo (Department of Otorhinolaryngology-Head and Neck Surgery, Inha University College of Medicine) ;
  • Cho Jung-Il (Department of Otorhinolaryngology-Head and Neck Surgery, Inha University College of Medicine) ;
  • Kim Yong-Jai (Department of Otorhinolaryngology-Head and Neck Surgery, Inha University College of Medicine) ;
  • Yang Tae-Yong (Department of Otorhinolaryngology-Head and Neck Surgery, Inha University College of Medicine) ;
  • Kim Dae-Hyung (Department of Otorhinolaryngology-Head and Neck Surgery, Inha University College of Medicine) ;
  • Park Chang-Sin (Department of Pharmacology, Inha University College of Medicine) ;
  • Han Chang-Jun (Department of Otorhinolaryngology, Kunkuk University College of Medicine)
  • 김영모 (인하대학교 의과대학 이비인후과학교실) ;
  • 조정일 (인하대학교 의과대학 이비인후과학교실) ;
  • 김용재 (인하대학교 의과대학 이비인후과학교실) ;
  • 양태용 (인하대학교 의과대학 이비인후과학교실) ;
  • 김대형 (인하대학교 의과대학 이비인후과학교실) ;
  • 박창신 (인하대학교 의과대학 약리학교실) ;
  • 한창준 (건국대학교 의과대학 이비인후과학교실)
  • Published : 2001.11.01
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Abstract

Background and Objectives: Nitric oxide (NO) is generated in mammalian tissue by the conversion of L-arginine to L-citrulline. This reaction is catalyzed by nitric oxide synthase (NOS). NO is an important bioactive agent and a signalling molecule that mediates a variety of biologic actions such as vasodilation, neurotransmission, host defense, and iron metabolism but increased NO production may also contribute to the pathogenesis of a various of disorders, including cancer. Before now, the role of NO in thyroid gland is still investigated and it was supposed that NO mediate the angiogenesis in tumor growth. Others journal and works identified the expression of iNOS that involve by neutrophil and eNOS that involve in part in the vascular remodeling and to understand the role of NO in human thyroid gland. But authors revealed only eNOS in thyroid neoplasm. iNOS was identifed by inflammation in fault. Materials and Methods: Western blot analysis was performed, using a polyclonal antibody against eNOS (Rabbit polyclonal IgG). Using the same antibody, the distribution of eNOS was examined in 15 formalin-fixed paraffin embedded samples by immunohistochemistry. By NADPH consumption rate, NOS activity was estimated at nodular hyperplasia. Results: Western blot analysis exhibited that eNOS was significantly elevated in thyroid papillary carcinoma, compared to that in nodular hyperplasia and normal tissue. Immunohistochemistry showed that the immunoreacitivity was present more significantly in thyroid follicular epithelial cell layer than vascular endothelial cell. NOS activity increased in nodular hyperplasia. Conclusions: Thyroid papillary cancer without neutrophil invasion expressed only eNOS. The endothelial localization of eNOS may play an important role in pathogenensis of human thyroid nodular hyperplasia and the follicular localization of thyroid papillary carcinomas.

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