결핵균 PPD, 30-kDa 및 TSP 항원에 의한 치료전 폐결핵환자 말초혈액 단핵구의 IL-12 및 TNF-α 생성능

IL-12 and TNF-α productions from human peripheral blood mononuclear cells in untreated patients with active pulmonary tuberculosis stimulated with 30-kDa or TSP antigen of Mycobacterium tuberculosis H37Rv

  • 송창화 (충남대학교 의과대학 미생물학교실) ;
  • 조은경 (충남대학교 의과대학 미생물학교실) ;
  • 이지숙 (충남대학교 의과대학 미생물학교실) ;
  • 김대수 (을지대학교 의과대학 내과학교실) ;
  • 임재현 (충남대학교 의과대학 미생물학교실) ;
  • 김운옥 (충남대학교 의과대학 미생물학교실) ;
  • 남현희 (충남대학교 의과대학 미생물학교실) ;
  • 김화중 (충남대학교 의과대학 미생물학교실) ;
  • 백태현 (건양대학교 의과대학 미생물학교실) ;
  • 박정규 (충남대학교 의과대학 미생물학교실)
  • Song, Chang-Hwa (Department of Microbiology, College of Medicine, Chungnam National University) ;
  • Jo, Eun-Kyeong (Department of Microbiology, College of Medicine, Chungnam National University) ;
  • Lee, Ji-Suk (Department of Microbiology, College of Medicine, Chungnam National University) ;
  • Kim, Dae-Su (Department of Internal Medicine, Eulji Medical College) ;
  • Lim, Jae-Hyun (Department of Microbiology, College of Medicine, Chungnam National University) ;
  • Kim, Un-Ok (Department of Microbiology, College of Medicine, Chungnam National University) ;
  • Nam, Hyeon-Hui (Department of Microbiology, College of Medicine, Chungnam National University) ;
  • Kim, Hwa-Jung (Department of Microbiology, College of Medicine, Chungnam National University) ;
  • Paik, Tae-Hyun (Department of Microbiology, College of Medicine, Konyang University) ;
  • Park, Jeong-Kyu (Department of Microbiology, College of Medicine, Chungnam National University)
  • 발행 : 2001.12.31

초록

To determine if initial infection with Mycobacterium tuberculosis changes the balance of cytokines between T cells and macrophages, we evaluated interferon (IFN)-${\gamma}$), interleukin-12 (IL)-12, and tumor necrosis factor (TNF)-${\alpha}$ productions by peripheral blood mononuclear cells (PBMC) from 15 untreated active pulmonary tuberculosis (TB) patients and 12 healthy tuberculin reactors (HTR). Freshly isolated PBMC were stimulated with Triton X-100 solubilized protein (TSP), 30-kDa or purified protein derivatives (PPD) antigen for 6, 18 and 96 hours. IL-12 p40 production by antigen-stimulated PBMC from TB patients was significantly decreased compared with that in HTR. In addition, IFN-${\gamma}$ production was significantly depressed in TB patients than that in HTR at a 96-hr stimulation. However, TNF-${\alpha}$ production was significantly higher in antigen-stimulated PBMC from TB than that of HTR. A pronounced increase in IFN-${\gamma}$ protein followed neutralization of IL-10 in early TB patients. However, neutralization of TNF-${\alpha}$ did not significantly alter IFN-${\gamma}$ induction in PBMC from TB patients. There were no significantly differences in the cytokine productions among three proteins, TSP, 30-kDa or PPD antigen. These results indicate that development of TB may be strongly associated with dysregulated productions of IL-12, IFN-${\gamma}$ and TNF-${\alpha}$, during the initial immune responses to M. tuberculosis. Further understanding of operative cytokine networks during human immune cell responses to protein antigens of M. tuberculosis may improve strategies for vaccine development.

키워드

과제정보

연구 과제 주관 기관 : 한국과학재단