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The Korean Association of Immunobiologists (대한면역학회)
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The effect of Korean mistletoe extract M11C (non-lectin components) on IL-1β release and expression from macrophages

Macrophages로부터 IL-1β 분비 및 전사에 있어서 한국산 겨우살이 추출물 M11C (non-lectin components)의 효과

  • Chang, Sung Ho (Department of Immunology, College of Medicine, Konkuk University) ;
  • Jun, Myung Ha (Department of Immunology, College of Medicine, Konkuk University) ;
  • Kang, Tae Bong (Department of Immunology, College of Medicine, Konkuk University) ;
  • Mun, Se Hwan (Department of Immunology, College of Medicine, Konkuk University) ;
  • Lee, Jun Ho (Department of Immunology, College of Medicine, Konkuk University) ;
  • Seong, Nak Sul (National Crop Experiment Station, RDA) ;
  • Lee, Sung Tae (Department of Orthopaedic Surgery, College of Medicine, Konkuk University) ;
  • Kim, Jong Bae (The Institute for Biomedical Research, Handong University) ;
  • Her, Erk (Department of Immunology, College of Medicine, Konkuk University)
  • 장성호 (건국대학교 의과대학 면역학교실) ;
  • 전명하 (건국대학교 의과대학 면역학교실) ;
  • 강태봉 (건국대학교 의과대학 면역학교실) ;
  • 문세환 (건국대학교 의과대학 면역학교실) ;
  • 이준호 (건국대학교 의과대학 면역학교실) ;
  • 성낙술 (농촌진흥청 작물시험장 특용작물과) ;
  • 이성태 (건국대학교 의과대학 정형외과학교실) ;
  • 김종배 (한동대학교 생의학 연구소) ;
  • 허억 (건국대학교 의과대학 면역학교실)
  • Published : 2001.08.31
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Abstract

Background: Korean mistletoe (Viscum album) extract has been found to posses immunostimulatory activity. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to know whether this extract might activate mouse peritoneal macrophages to produce interleukin $1{\beta}$ (IL-$1{\beta}$). Methods: Hemagglutination assay was carried out to examine whether M11C contained a lectin or not. To know the effect of M11C on the production of IL-$1{\beta}$, the macrophages were treated by the M11C, and then collected the supernatant (M11C stimulated macrophages-conditioned media; MMCM). MMCM was analyzed for the IL-$1{\beta}$ quantification and mRNA expression by means of ELISA and RT-PCR, respectively. Results: Maximum effective dose and time of M11C on IL-$1{\beta}$ production from macrophages were $20{\mu}g/m{\ell}$ and 8 hours, respectively. This ELISA data was reconfirmed by immunoblotting assay. indicating that M11C is a good candidate for an immunomodulator. The dose and time dependent effects of M11C on the expression of IL-$1{\beta}$ mRNA from macrophages was also shown in expression of mRNA detected by RT-PCR. Treatment dose and time for the maximum expression of IL-$1{\beta}$ mRNA were $20{\mu}g/m{\ell}$ and 4 hours, respectively. Maximum gene expression of IL-$1{\beta}$ was much earlier than maximum production of it. Conclusion: As results, Korean mistletoe extract, M11C, may be used for an immunomodulator. This will be able to make up for and solve the problems caused by existent immunoagent with many adverse effects through many other studies in future including one molecule extraction.

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