Journal of Korean Neurosurgical Society

The Korean Neurosurgical Society (대한신경외과학회)
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Neural Antigen Expressions in Cultured Human Umbilical Cord Blood Stem Cells in vitro

시험관내 배양된 제대혈 모세포에서의 신경항원 발현

  • Ha, Yoon (Department of Neurosurgery, Seoul District Hospital) ;
  • Yoon, Do Heum (Department of Neurosurgery, Yonsei University, College of Medicine) ;
  • Yeon, Dong Su (Institute of Brain Research, Yonsei University, College of Medicine) ;
  • Kim, Hyun Ok (Department of Pathology, Yonsei University, College of Medicine) ;
  • Lee, Jin Ju (Institute of Brain Research, Yonsei University, College of Medicine) ;
  • Cho, Yong Eun (Department of Neurosurgery, Yonsei University, College of Medicine) ;
  • Choi, Joong Uhn (Department of Neurosurgery, Yonsei University, College of Medicine)
  • 하윤 (국군 서울 지구병원 신경외과) ;
  • 윤도흠 (연세대학교 의과대학 신경외과학교실) ;
  • 연동수 (연세대학교 의과대학 뇌연구소) ;
  • 김현옥 (연세대학교 의과대학 임상병리학교실) ;
  • 이진주 (연세대학교 의과대학 뇌연구소) ;
  • 조용은 (연세대학교 의과대학 신경외과학교실) ;
  • 최중언 (연세대학교 의과대학 신경외과학교실)
  • Received : 2001.04.27
  • Accepted : 2001.07.02
  • Published : 2001.08.28
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Abstract

Objectives : Cord blood stem cells have been widely used as donor cells for bone marrow transplantation recently. These cells can give rise to a variety of hematopoietic lineages to repopulate the blood. Recent observations reveal that some bone marrow cells and bone marrow stromal cells(MSCs) can grow to become either neurons or glial cells. It is, however, unclear whether or not there exists stems cells which can differentiate into neurons in the blood during the early stages of postnatal life. Methods : Human cord blood stem cells were prepared from human placenta after full term delivery. To induce neuronal differentiation of stem cells, ${\beta}$-mercaptoethanol was treated. To confirm the neuro-glial characteristics of differentiated stem cells, immunocytochemical stain for NeuN, neurofilament, glial fibrillary acidic protein(GFAP), microtubule associated protein2(MAP2) was performed. RT-PCR was performed for detecting nestin mRNA and MAP2 mRNA. Results : We showed in this experiment that neuro-glial markers(NeuN, neurofilament, MAP2, GFAP) were expressed and axon-like cytoplasmic processes are elaborated in the cultured human cord blood stem cells prepared from new born placenta after full term delivery. Nestin mRNA was also detected in fresh cord blood monocytes. Conclusions : These results suggest that human cord blood derived stem cells may be potential sources of neurons in early postnatal life.

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