Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)
- Volume 34 Issue 5
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- Pages.556-562
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- 2001
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- 0374-8111(pISSN)
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- 2287-8815(eISSN)
Structure and Isolation of Antioxidative Substance Derived from Phaeodactylum tricornutum
Phaeodactylum tricornutum으로부터 항산화성 물질의 분리 및 구조
- KIM Se-Kwon (Department of Chemistry, Pukyong National University) ;
- BYUN Hee-Guk (Department of Chemistry, Pukyong National University) ;
- BAEK Ho-Chul (Department of Chemistry, Pukyong National University) ;
- PARK Pyo-Jam (Department of Chemistry, Pukyong National University) ;
- KANG Ok-Ju (School of Food Science, Dong-Ju College) ;
- KIM Jong-Bae (Department of Food Science and Technology, Kunsan National University)
- 김세권 (부경대학교 화학과) ;
- 변희국 (부경대학교 화학과) ;
- 백호철 (부경대학교 화학과) ;
- 박표잠 (부경대학교 화학과) ;
- 강옥주 (동주대학 식품과학계열) ;
- 김종배 (군산대학교 식품공학과)
- Published : 2001.09.01
Abstract
The antioxidative activity of marine microalgae, Phaeodactylum tricornutum (P. tricornutum) of Bacillariophyceae, was determined by measuring radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The chloroform fraction of P. tricornutum showed strong antioxidative activity, The potential antioxidative activity of factions extracted with mixture solution of organic solvents was detected in dichloromethane : methanol (2 : 1) fraction, This fraction was further purified by preparative thin layer chromatography (PTLC) and repeated reverse-phase HPLC. On the basis of chemical and spectoscopic evidence from results obtained by UV, FT-IR, EIMS and NMR, the compound purified from P. tricornutum was identified as zeaxanthin.
본 연구에서는 비교적 성장이 빠르고 배양이 용이한 황갈편조 식물문 중 규조강에 속하는 P. tricornutum으로부터 선정하여 항 산화성 물질을 분리