한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)
- 제34권5호
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- Pages.502-508
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- 2001
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- 0374-8111(pISSN)
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- 2287-8815(eISSN)
Vibrio furnissii로부터 세포외 용혈소의 정제 및 특성
Purification and Characterization of the Extracellular Hemolysin of Vibrio furnissii
- 허문수 (제주대학교 해양생산과학부)
- HEO Moon-Soo (Faculty of Applied Marine Sciences, Cheju National University)
- 발행 : 2001.09.01
초록
병원성 비브리오 속인 Vibrio furnissii의 용혈소를 분리정제하고 그 물리화학적 특성, 생물학적인 그리고 면역학적인 상호관련성을 검토하여, 다음과 같은 결과를 얻었다. 용혈소의 정제는 ammonium sulfate precipitation, DEAE-Sephadex A-50 (1st와 2nd)와 Sephaedx G-100 gel permeation chromatography를 거쳐 738배로 정제되었으며 회수율은 각각
The extracellualr hemolysin produced by pathogenic Vibrio furnissii was isolated and purified by ammonium sulfate precipitation and followed by DEAE-Sephadex A-50 (1st and 2nd) ion-exchange and Sephadex G-100 gel permeation chromatography. The molecular weight of the purified hemolysin was 61 kDa. The enzyme activity was inactivated by heating at 60 for 5 min, and inhibited by additions of