대한수의학회지 (Korean Journal of Veterinary Research)

  • 제41권1호
  • /
  • Pages.51-57
  • /
  • 2001
  • /
  • 2466-1384(pISSN)
  • /
  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지

부루세라 RB51의 ELISA 진단법 개발 II. Brucella abortus RB51균의 8kDa 항원 정제 및 ELISA 진단법 개발

Development of ELISA for Brucella abortus RB51 II. Purification of 8kDa antigen and development of ELISA using its antigen of Brucella abortus RB51

  • 허문 (국립수의과학검역원 세균과) ;
  • 조동희 (국립수의과학검역원 세균과) ;
  • 정병열 (국립수의과학검역원 세균과) ;
  • 조성근 (국립수의과학검역원 세균과) ;
  • 정석찬 (국립수의과학검역원 세균과) ;
  • 김옥경 (국립수의과학검역원 세균과)
  • Her, Moon (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Services) ;
  • Cho, Dong-hee (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Services) ;
  • Jung, Byeong-yeal (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Services) ;
  • Cho, Seong-kun (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Services) ;
  • Jung, Suk-chan (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Services) ;
  • Kim, Ok-kyung (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Services)
  • 심사 : 2000.12.04
  • 발행 : 2001.03.20
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초록

A procedure for extraction and purification of 8 kDa antigen of Brucella abortus RB51 was developed. Bacteria heat inactivated at $60^{\circ}C$, 30 min was extracted by 1% sarcosine and followed by fluid pressure liquid gel filtration chromatography of 2 series, Superose 12 HR 10/30 and Sephacryl S-100. There was produced $71.46{\mu}g/g$(wet) of 8 kDa antigen, and it resisted 1% trypsin, solved 1% triton X-100 higher than distilled water and inactivated 0.1% proteinase K. These results show that 8 kDa antigen may be a lipoprotein existed cell surface of B. abortus RB51. Also, we developed ELISA using purified 8 kDa surface antigen of Brucella abortus RB51 strain, its specificity and sensitivity was 95.0%, 98.6%, respectively. As compared with dot-blot assay using whole cell and ELISA using 8 kDa antigen, its correlation was 93.5%.

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