Korean Journal of Veterinary Research (대한수의학회지)
- Volume 41 Issue 3
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- Pages.381-385
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- 2001
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Establishment of diagnositc method for bovine neosporosis by PCR using paraffin block
파라핀 블록 PCR을 이용한 소 네오스포라 감염증의 진단법 확립
- Lee, Jung-keun (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
- Kim, Jae-Hoon (National Veterinary Research Institute and Quarantine Service) ;
- Kim, Jin-Hyun (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
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Lee, Byung-chun
(College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
- Hwang, Woo-suk (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
- Youn, Hee-jeong (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
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Nam, Hoo-woo
(Department of Parasitology, College of Medicine, Catholic University) ;
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Jean, Young-wha
(National Veterinary Research Institute and Quarantine Service) ;
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Kim, Dae-Yong
(College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
- 이중근 (서울대학교 수의과대학, 농생명공학부) ;
- 김재훈 (국립수의과학검역원) ;
- 김진현 (서울대학교 수의과대학, 농생명공학부) ;
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이병천
(서울대학교 수의과대학, 농생명공학부) ;
- 황우석 (서울대학교 수의과대학, 농생명공학부) ;
- 윤희정 (서울대학교 수의과대학, 농생명공학부) ;
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남호우
(카톨릭의과대학 기생충학교실) ;
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진영화
(국립수의과학검역원) ;
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김대용
(서울대학교 수의과대학, 농생명공학부)
- Accepted : 2001.08.23
- Published : 2001.09.25
Abstract
Neospora caninum infections have been associated with neonatal paresis as well as abortion around the world. Bovine abortion induced by N caninum was first reported in 1997 in Korea. Diagnosis of N caninum infection is usually based on histopathology and immunohistochemical detection of organism. However, often the tissues having lesion suggestive of N caninum infection were negative on immunohistochemistry. Here, we describe establishment of PCR-based diagnostic strategy for N caninum infection using DNA extracted from paraffin blocks containing the lesion. PCR was able to amplify N caninum-specific bands from the paraffin blocks containing at least moderate degree of inflammation. Compared to paraffin-blocks, DNA extracted from fresh tissues were less sensitive than that of paraffin blocks. This PCR-based method can be practically applicable for rapid diagnosis of bovine N caninum infection with high specificity and sensitivity. Based on this method, 17% of bovine abortion surveyed during a designated period was associated with N caninum infection.