Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지

Isolation, Identification and Determination of Antioxidant in Ginger (Zingiber officinale) Rhizome

  • Cho, Kang-Jin (Division of Biochemistry, National Institute of Agricultural Science and Technology) ;
  • Kim, Jin-Weon (Division of Biomaterial Utilization, National Institute of Agricultural Science and Technology) ;
  • Choi, In-Lok (Division of Upland Crops, National Honam Agricultural Experiment Station) ;
  • Kim, Jung-Bong (Division of Biochemistry, National Institute of Agricultural Science and Technology) ;
  • Hwang, Young-Soo (Division of Biochemistry, National Institute of Agricultural Science and Technology)
  • Received : 2000.10.06
  • Published : 2001.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The antioxidative compounds and antioxidant contents of ginger (Zingiber officinale) rhizomes were determined. Substances reextracted using ethyl acetate from crude methanol extract of fresh ginger rhizome were separated through thin layer chromatography. Ten phenolic antioxidative bands were visualized through color reactions using ferric chloride-potassium ferricyanide and 1,1-diphenyl-2-picrylbydrazyl (DPPH). The antioxidative compounds were purified through preparative TLC and high performance liquid chromatography (HPLC), among which, five antioxidants were identified as 4-, 6-, 8-. and 10-gingerols and 6-shogaol on the basis of their molecular weights determined through LC-MS. As shown in experiments using DPPH free radicals, 6-Gingerol and PT4-HP8 (unknown) were revealed to be more efficient than BHT (butylated hydroxy toluene). Contents of gingerols were determined through reverse phase HPLC. Total gingerol contents (sum of 6-,8-, and 10-gingerols) in rhizomes of different ginger varieties varied significantly. The HG55 (collected at Wanju district in Korea) and the HG52 (imported from Brazil) showed the highest gingerol contents.

Keywords