Journal of Applied Biological Chemistry
- Volume 44 Issue 2
- /
- Pages.59-64
- /
- 2001
- /
- 1976-0442(pISSN)
- /
- 2234-7941(eISSN)
Molecular Cloning and Characterization of Sesquiterpene Cyclase cDNAs from Pepper Plant Infected with Phytophthora capsici
- Kim, Jong-Bum (Division of Biochemistry, Department of Biological Resources, National Institute of Agricultural Science and Technology) ;
-
Lee, Sung-Gon
(Division of Biochemistry, Department of Biological Resources, National Institute of Agricultural Science and Technology) ;
- Ha, Sun-Hwa (Division of Biochemistry, Department of Biological Resources, National Institute of Agricultural Science and Technology) ;
- Lee, Myung-Chul (Division of Cellular Genetics, Department of Biological Resources, National Institute of Agricultural Science and Technology) ;
- Ye, Wan-Hye (Division of Plant Pathology, Department of Crop Protection, National Institute of Agricultural Science and Technology) ;
- Lee, Jang-Yong (Division of Rice Breeding, Crop Experimental Station) ;
- Lee, Shin-Woo (Division of Biochemistry, Department of Biological Resources, National Institute of Agricultural Science and Technology) ;
- Kim, Jung-Bong (Division of Biochemistry, Department of Biological Resources, National Institute of Agricultural Science and Technology) ;
- Cho, Kang-Jin (Division of Biochemistry, Department of Biological Resources, National Institute of Agricultural Science and Technology) ;
- Hwang, Young-Soo (Division of Biochemistry, Department of Biological Resources, National Institute of Agricultural Science and Technology)
- Received : 2001.05.07
- Published : 2001.06.30
Abstract
Pepper plants (Nogkwang, 60-day old) were inoculated with Phytophthora capsici to induce sesquiterpene cyclase associated with the biosynthesis of phytoalexin (capsidiol), a substance related to the defense against pathogens in plants. One day after inoculation, mRNA was isolated from the root, cDNA synthesized, and a library constructed in a ZAP express XR vector. The efficiency was