Journal of Periodontal and Implant Science
- Volume 31 Issue 4
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- Pages.833-846
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- 2001
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- 2093-2278(pISSN)
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- 2093-2286(eISSN)
The effect of safflower seed fraction extract on periodontal ligament fibroblast and MC3T3-E1 cell in vitro
홍화씨 분획 추출물이 치주인대 섬유아세포와 MC3T3-E1 세포에 미치는 영향
- Huh, Ji-Sun (Department of Periodontology, College of Dentistry, Yonsei University, Reasearch Institute for Periodontal Regeneration) ;
- Kang, Jung-Hwa (Department of Oral Biology, College of Dentistry, Yonsei University) ;
- Yoo, Yun-Jung (Department of Oral Biology, College of Dentistry, Yonsei University) ;
- Kim, Chang-Sung (Department of Periodontology, College of Dentistry, Yonsei University, Reasearch Institute for Periodontal Regeneration) ;
- Cho, Kyoo-Sung (Department of Periodontology, College of Dentistry, Yonsei University, Reasearch Institute for Periodontal Regeneration) ;
- Choi, Seong-Ho (Department of Periodontology, College of Dentistry, Yonsei University, Reasearch Institute for Periodontal Regeneration)
- 허지선 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
- 강정화 (연세대학교 치과대학 구강생물학교실) ;
- 유윤정 (연세대학교 치과대학 구강생물학교실) ;
- 김창성 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
- 조규성 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
- 최성호 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소)
- Published : 2001.12.31
Abstract
Recently, use of natural medicine is getting more attention, and some of them are believed to be effective in the treatment of periodontitis. Among them, the seeds of safflower(Carthamus tinctrorius L.) have been proven to be effective through its use in bone diseases such as fracture and osteoporosis. During the last few years, studies using the seeds of safflower gown in Korea have been active, and it has been reported that safflower seed extract increase the proliferation and the alkaline phosphatase(ALP) activity of human periodontal ligament fibroblast(hPDLF), osteoblast, and that they promote the mineralization process. In animal studies, when safflower seed extract were administered orally new bone formation was promoted. Recently, in an effort to find out the most effective osteogenic components, among many components of the safflower seed, various safflower seed fraction extracts were obtained by multistep extraction of the safflower components using various solvents. Among these, saf-M-W fraction extracted by methanol and water was most effective in increasing osteogenic potential of osteoblasts. In this study, the effect of safflower seed fraction extract, saf-M-W, on the growth and differentiation of hPDLF and MC3T3-E1 cell was investigated. The toxicity of saf-M-W on both cells was measured using M'IT(3-(4,5dimethylthiazol-2-y1)-2,5-diphenyl tetrazolium bromide) test, and ALP activity was measured using the colorimetric assay of hPDLF. In addition, in MC3T3-El cells, the expression of ALP, bone sialoprotein(BSP) mRNA was observed using Northern blot, and the mineralized nodule formation Was observed using von Kossa stain and phase-contrast microscope. 1. In concentrations below