Journal of Periodontal and Implant Science
- Volume 31 Issue 1
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- Pages.21-41
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- 2001
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- 2093-2278(pISSN)
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- 2093-2286(eISSN)
Effect of Citric Acid and Tetracycline HCl Root Conditioning on rhBMP-2 on Human Periodontal Ligament Fibroblast and Osteoblast cell
구연산과 테트라싸이클린으로 처리한 치근면에서 rhBMP-2가 치주인대섬유아세포와 골아세포의 활성에 미치는 영향
- Shim, Jung-Min (Department of Periodontology and Dental Research Institute, college of Dentistry, Seoul National University) ;
- Han, Soo-Boo (Department of Periodontology and Dental Research Institute, college of Dentistry, Seoul National University) ;
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Seol, Yang-Jo
(Department of Periodontology and Dental Research Institute, college of Dentistry, Seoul National University, Department of Dentistry, College of Medicine, Sungkyunkwan University) ;
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Lee, Yong-Moo
(Department of Periodontology and Dental Research Institute, college of Dentistry, Seoul National University) ;
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Kim, Kyeong-Hwa
(Department of Periodontology and Dental Research Institute, college of Dentistry, Seoul National University) ;
- Kye, Seung-Beom (Department of Dentistry, College of Medicine, Sungkyunkwan University) ;
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Choi, Sang-Mook
(Department of Periodontology and Dental Research Institute, college of Dentistry, Seoul National University) ;
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Chung, Chong-Pyoung
(Department of Periodontology and Dental Research Institute, college of Dentistry, Seoul National University)
- 심정민 (서울대학교 치과대학 치주과학교실 및 치학연구소) ;
- 한수부 (서울대학교 치과대학 치주과학교실 및 치학연구소) ;
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설양조
(서울대학교 치과대학 치주과학교실 및 치학연구소, 성균관대학교 의과대학 치과학교실) ;
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이용무
(서울대학교 치과대학 치주과학교실 및 치학연구소) ;
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김경화
(서울대학교 치과대학 치주과학교실 및 치학연구소) ;
- 계승범 (성균관대학교 의과대학 치과학교실) ;
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최상묵
(서울대학교 치과대학 치주과학교실 및 치학연구소) ;
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정종평
(서울대학교 치과대학 치주과학교실 및 치학연구소)
- Published : 2001.03.30
Abstract
The goal of Periodontal treatment is predictable periodontal regeneration. But until now, many products including GTR materials and growth factors are beyond of complete regeneration. BMP can induce ectopic bone formation when implanted into sites such as rat muscle and can greatly enhance healing of bony defects when applied exogenously. BMP can promote periodontal regeneration by their ability to stimulate new bone and new cementum formation. But little is known about optimal conditions required for the application. Root conditioning is used for bioacive root change so altered root surface provides a substrate that promotes chemotaxis, migration and attachment of peridontal cells encouraging connective attachment to the denuded root surface. The aim of this study is to investigate whether the acid conditioning change effect of rhBMP-2 on human periodontal ligament cell and osteoblast cell line. 288 periodontally involved root dentin slices are divided into 6 groups, each 48, 1)control, 2)treated with BMP, 3)treated with citric acid 4)treated with citric acid+BMP 5)treated with tetracycline 6)treated with TC+BMP. Each group was devided half, so 12 root dentin slices were seeded with periodontal ligament cells and 12 were seeded with osteoblasts. At day 2 and 7, cell number, protein assay, ALP activitiy was measured. To investigate morphology of cultured cells, SEM was employed. Statistical analysis was performed with SPSS 8.0 either t-test or ANOVA test. The results are ; Protein assay and cell number was slightly decreased in CA+BMP group compared to Ca group but it was not statistically significant and ALP activity was much more increased in CA+BMP group compared to CA group so there was no statistically significance between BMP and CA+BMP group and statistically significant compared to control group. Cell number and protein assay was slightly increased in TC group and ALP activity was much less the BMP group and CA group. Cell number and protein and ALP activity was not much increased in TC+BMP group. TC group and TC+BMP group showed cell morphology change in SEM. This results suggested that application of root surface with citric acid before BMP treatment might give better result in periodontal regeneration.
Keywords