The Korean Journal of Cytopathology (대한세포병리학회지)
- Volume 12 Issue 1
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- Pages.25-30
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- 2001
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- 1017-0391(pISSN)
Polymerase Chain Reaction Detection of Mycobacterium tuberculosis and Fine Needle Aspiration Cytology for the Diagnosis of Tuberculous Lymphadenitis
결핵성 림프절염의 진단를 위한 세침흡인 세포검사 및 중합효소연쇄 반응과 효소면역법을 이용한 Mycobacterium tuberculosis의 검출
- Kim, Joo-Heon (Department of Pathology, Eulji University School of Medicine) ;
- Kim, Nam-Hoon (Department of Pathology, Eulji University School of Medicine) ;
- Kang, Dong-Wook (Department of Pathology, Eulji University School of Medicine) ;
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Park, Mee-Ja
(Department of Pathology, Eulji University School of Medicine) ;
- Moon, Sang-Kyoung (Department of Pathology, Eulji University School of Medicine) ;
- Yu, Tae-Cho (Department of Pathology, Eulji University School of Medicine) ;
- Jang, Eun-Ju (Department of Pathology, Eulji University School of Medicine)
- 김주헌 (을지대학교 의과대학 병리학교실) ;
- 김남훈 (을지대학교 의과대학 병리학교실) ;
- 강동욱 (을지대학교 의과대학 병리학교실) ;
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박미자
(을지대학교 의과대학 병리학교실) ;
- 문상경 (을지대학교 의과대학 병리학교실) ;
- 유태조 (을지대학교 의과대학 병리학교실) ;
- 장은주 (을지대학교 의과대학 병리학교실)
- Published : 2001.06.30
Abstract
Tuberculous lymphadenitis is not uncommon in Korea. Therefore, an inexpensive, safe and rapid method is needed to diagnose the tuberculous lymphadenitis. Flne needle aspiration cytology Is a good method for this purpose, but has several limitations in the diagnosis of tuberculous lymphadenitis, especially when the presence of acid-fast bacilli is not proved. To evaluation the usefulness of the polymerase chain reaction with enzyme immunoassay technique in the detection of Mycobacterium tuberculosis (M. tuberculosis) In the cervical Iymph node asplrates, the authors performed fine needle aspiration cytology and M. tuberculosis PCR with enzyme immunoassay for mycobacterial DNA sequences from 15 cases of the fine needle aspirates. Cytomorphologically, the cases were categorized into three types: predominantly necrotic materials; typical epithelioid cell granulomas with or without slant cells and caseous necrosis; and non-tuberculous lesions, such as reactive lymphadenitis, abscess, metastatic carcinoma and malignant lymphoma. M. tuberculosis DNA was found in 8 of 15 cases by PCR with enzyme immunoassay. Negative findings on PCR were achieved in 7 cases, which revealed non-tuberculous tymphadenopathy. In conclusion, we suggest that M. tuberculosis PCR with enzyme immunoassay using the fine needle aspirates is a very useful tool for the diagnosis of tuberculous lymphadenitis.