Ultrastructural Study of the Liver by Chitosanoligosaccharide Administrated in Rat

키토산올리고당을 섭취한 쥐에서 간 미세구조의 연구

The purpose of this study was to evaluate the histological toxicity of chitosanoligosaccharide on the rat. A healthy male of Wistar rat that weighted $250{\pm}350g$ was used for experiment The experimental group was divided into five groups. Group 1 was control group which treated with general food Group 2 was $F_1$ generation which was born by mating of 0.1% (1 mg/ml) chitosanoligosaccharide was supplied by feeding ad libitum for 30 days. Group 3 was $F_2$ generation which was born by mating $F_1$ generation. Group 4 was treated with 90 days of 0.1% (1 mg/ml) chitosan oligosaccharide. Group 5 was treated with 365 days of 0.1% (1 mg/ml) chitosanoligo saccharide. All experimental groups were used to 10 rat. The results were as follow: The RER dilation was observed Group 4. However, there were no significantly changes of ultrastructures in the other groups compared to the control. It was concluded that chitosanoligosaccharide can be used for nontoxic natural material.

본 연구의 목적은 키토산올리고당의 쥐 간조직 독성여부를 관찰하고자 하였다. 건강한 Wistar계 쥐를 사용하였다. 실험군은 Group 1. 일반식이를 섭취한 대조군, Group 2 0.1%(1mg/ml)키토산올리고당 수용액을 30일 간 음용수를 통해 자유자재로 섭취시킨 후 교미시켜 태어난 $F_1$ 세대, Group 3 $F_1$ 세대의 쥐에게 0.1%(1 mg/ml) 키토산올리고당 수용액을 30일간 음용수를 통해 자유자재로 섭취한 후 교미시켜 태어난 $F_2$ 세대, Group 40.1% (1 mg/ml) 키토산올리고당 수용액을 90일간 음용수를 통해 자유자재로 섭취시킨 군, Group 50.1% (1 mg/ml) 키토산올리고당 수용액을 365일간 음용수를 통해 자유자재로 섭취시킨 군의 쥐 등으로 각 실험군 당 쥐 10마리를 사용하였고, 다음과 같은 결과를 얻었다. Group 4의 경우 약간의 소포체 팽창을 관찰하였을뿐, 다른 실험군에서는 대조군과 비교하여 특별한 간 조직의 미세구조 변화를 관찰하지 못하였다. 결론적으로 키토산올리고당은 무독성 물질로서 안전성이 있다고 사료된다.