Applied Microscopy
- Volume 31 Issue 3
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- Pages.275-285
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- 2001
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- 2287-5123(pISSN)
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- 2287-4445(eISSN)
Increase of Alveolar Macrophages Contributes to the Enhanced Xanthine Oxidase Activity in the Bronchoalveolar Lavage Fluid of Rats Given IL-1 Intratracheally
Interleukin-1의 기관지 투여 후 나타나는 폐세척액 내 대식세포의 수적변화에 따른 Xanthine Oxidase의 활성변화
- Cho, Hyun-Gug (Dept. of Visual Optics, Kyungwoon University) ;
- Yoon, Chong-Guk (Dept. of Public Health, Keimyung University) ;
- Choi, Jeung-Mok (Dept. of Biology, Yeungnam University) ;
- Park, Won-Hark (Dept. of Biology, Yeungnam University) ;
- Lee, Young-Man (Dept. of Physiology, School of Medicine, Daegu Catholic University)
- 조현국 (경운대학교 안경광학과) ;
- 윤종국 (계명대학교 공중보건학과) ;
- 최정목 (영남대학교 생물학과) ;
- 박원학 (영남대학교 생물학과) ;
- 이영만 (대구가톨릭대학교 의과대학 생리학교실)
- Published : 2001.09.01
Abstract
The pulmonary alveolar macrophage is thought to play an important role in the mediation of acute inflammatory lung injury by secretory products including degraded enzymes, cytokines, and reactive oxygen metabolites . This study was conceived to understand the role of alveolar macrophage in oxidative stress induced acute lung injury. To examine the alveolar macrophages and xanthine oxidase (XO) activity in bronchoalveolar lavage fluid (BALF), time-dependent changes of numbers of alveolar macrophages, monocytes and neutrophils in alveolar cavity were counted in association with ultrastructural and cytochemical observations of lung tissue and alveolar cells. The number of monocytes was increased (p<0.001) at 1h after IL-1 treatment compared with that of sham. At 2h after instillation of IL-1, the number of alveolar macrophages was the highest, XO activity in BALF was elevated at 2h after IL-1 instillation and the activity was markedly elevated(p<0.05) at 3h after IL-1 treatment. On the basis of these experimental results, it is suggested that, during early phase of acute lung injury induced by IL-1, alveolar macrophage-derived XO contributes to lung injury earlier than the neutrophilic respiratory burst.
폐포강 대식세포는 사이토카인, 유해산소 대사물을 포함한 그들이 분비하는 물질들로 인해 급성 폐손상에 있어서 직접, 간접적으로 폐손상의 초기반응에 중요한 역할을 담당하는 것으로 알려져 있다. 본 연구에서는