Mycobiology
- 제29권3호
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- Pages.132-134
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- 2001
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- 1229-8093(pISSN)
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- 2092-9323(eISSN)
Improved Transformation of the Filamentous Fungus Aspergillus niger Using Agrobacterium tumefaciens
- Park, Seung-Moon (Basic Science Research Institute, Chonbuk National University)
- 발행 : 2001.09.30
초록
Since it is known that Agrobacterium tumefaciens, which has long been used to transform plants, can transfer the T-DNA to yeast Saccharomyces cerevisiae during tumourigenesis, a variety of fungi were subjected to transformation to improve their transformation frequency. In this study, I report the A. tumefaciens-mediated transformation of filamentous fungus Aspergillus niger. Transfer of the binary vector pBIN9-Hg, containing the bacterial hygromycin B phosphotransferase gene under the control of the Aspergillus nidulans trpC promoter and terminator as a selectable marker, led to the selection of